• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chinese science bulletin >Study on cellular internalization of poly(vinyldiaminotriazine)-based hydrogen bonding type non-viral transgene vector
【24h】

Study on cellular internalization of poly(vinyldiaminotriazine)-based hydrogen bonding type non-viral transgene vector

机译：基于聚（乙烯基二氨基三嗪）的氢键型非病毒转基因载体的细胞内在化研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Previously we successfully prepared poly(vinyldiaminotriazine)(PVDT)-based non-viral vectors which complexed plasmid DNA via hydrogen bonding with adenine-thymine base pairs. In this report, surface charges and complex sizes of this system were further examined. The results showed that PVDT-based polymer could cover surface charges of DNA resulting in slightly negative or neutral complexes. It was also found that the complex sizes were governed by two events: the aggregation induced by the instability of neutral particles, and more compact complexes produced by PVDT-based polymers. In the study of cellular uptake, chlorpromazine and filipin III were used to inhibit clathrin- and caveolae-mediated endocytosis, respectively. We found that PVDT-based systems were transported into cells via a non-clathrin, non-caveolae mediated endocytosis. This special process was studied by temperature inhibition and kinetics assays. It was revealed that such a pathway was characterized by (i) a more energy dependent process and (ii) a much slow transfection-effective internalization.
机译：以前，我们已经成功地制备了基于聚（乙烯基二氨基三嗪）（PVDT）的非病毒载体，该载体通过与腺嘌呤-胸腺嘧啶碱基对的氢键作用来复合质粒DNA。在此报告中，进一步检查了该系统的表面电荷和复杂尺寸。结果表明，基于PVDT的聚合物可以覆盖DNA的表面电荷，从而产生轻微的负或中性复合物。还发现复合物的大小受两个事件控制：中性粒子的不稳定性引起的聚集，以及基于PVDT的聚合物产生的更紧密的复合物。在细胞摄取研究中，氯丙嗪和菲林III分别用于抑制网格蛋白和小窝介导的内吞作用。我们发现基于PVDT的系统通过非clathrin，非caveolae介导的内吞作用被转运到细胞中。通过温度抑制和动力学分析研究了这一特殊过程。揭示了这种途径的特征在于（i）更多的能量依赖性过程和（ii）非常慢的转染有效内在化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号