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首页> 外文期刊>Chinese science bulletin >Human RBCs blood group conversion from A to O using a novel alpha-N-acetylgalactosaminidase of high specific activity
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Human RBCs blood group conversion from A to O using a novel alpha-N-acetylgalactosaminidase of high specific activity

机译：使用高比活性的新型α-N-乙酰半乳糖苷酶将人类RBC的血型从A转换为O

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摘要

alpha-N-acetylgalactosaminidase (alpha NAGA) can convert group A human red blood cells (RBCs) to group O. One novel alpha NAGA gene was cloned by PCR from Elizabethkingia meningosepticum isolated from a domestic clinical sample. Pure recombinant alpha NAGA was obtained by genetic engineering and protein purification with a calculated molecule of 49.6 kD. alpha NAGA was selective for terminal alpha-N-acetylgalactosamine residue with a high specific activity. alpha NAGA could completely remove A antigens of 1 U (about 100 mL) group A(1) or A(2) RBCs in 1 h at pH 6.8 and 25 degrees C with a consumption of 1.5 or 0.4 mg recombinant enzyme. Enzyme-converted group A RBCs did not agglutinate after being mixed with monoclonal anti-A or sera of groups A, B, AB and O. Other blood group antigens except ABO had no change. FCM analysis showed that A antigens and A(1) antigens disappeared while H antigens increased. It indicated that alpha NAGA successfully converted human blood group A RBCs to universally transfusable group O RBCs without the risk of ABO-incompatible transfusion reactions. This alpha NAGA was suitable for producing universal RBCs to increase clinical transfusion safety, improve the RBCs supply, and to decrease transfusion cost and support transfusion service in case of emergency.
机译：α-N-乙酰半乳糖苷酶（αNAGA）可以将A组人类红细胞（RBC）转换为O组。通过PCR分离了一个从国内临床样本中分离出的脑膜炎伊利沙伯氏菌，这是一个新的αNAGA基因。通过基因工程和蛋白质纯化，以49.6 kD的计算分子获得纯的重组αNAGA。 αNAGA对具有高比活性的末端α-N-乙酰半乳糖胺残基具有选择性。 αNAGA可以在pH 6.8和25摄氏度下于1小时内完全清除1 U（约100 mL）A（1）或A（2）RBCs组的A抗原，消耗1.5或0.4 mg重组酶。与单抗A或A，B，AB和O组的血清混合后，经酶转化的A组RBC不会凝集。除ABO以外的其他血型抗原均无变化。 FCM分析表明，A抗原和A（1）抗原消失，而H抗原增加。这表明αNAGA成功地将人类血液A组红细胞转化为可普遍输血的O组红细胞，而没有发生ABO不相容输血反应的风险。这种alpha NAGA适用于生产通用的RBC，以提高临床输血安全性，改善RBC的供应量，降低输血成本并在紧急情况下支持输血服务。

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