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首页> 外文期刊>Chinese science bulletin >Rescued virus from infectious cDNA clone of rabbit hemorrhagic disease virus is adapted to RK13 cells line
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Rescued virus from infectious cDNA clone of rabbit hemorrhagic disease virus is adapted to RK13 cells line

机译：兔出血性疾病病毒感染性cDNA克隆中拯救的病毒适用于RK13细胞系

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摘要

Based on the infectious full-length cDNA clone of rabbit hemorrhagic disease virus (RHDV), the in vitro transcripts are introduced into RK13 cells, 12 h later, CPE could be observed clearly, and virual antigen could also be detected by IFA. The titre of the recovered virus is 10~(4.6)/mL. Immune electron microscopic observation of the virus particles revealed that the particles were rotund with a diameter of about 30 nm. Besides, virus titre quantification obtained by qRT-PCR showed a correlation between time from infection and virus titre. All these results showed that we have recovered RHDV from RK13 cells by reverse genetics technology successfully, and this would be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and new type vaccines of RHDV.
机译：根据兔出血性疾病病毒（RHDV）的感染性全长cDNA克隆，将体外转录本导入RK13细胞，在12 h后，可以清楚地观察到CPE，并且通过IFA也可以检测到病毒抗原。回收的病毒滴度为10〜（4.6）/ mL。病毒颗粒的免疫电子显微镜观察表明，该颗粒是圆形的，直径约为30nm。此外，通过qRT-PCR获得的病毒滴度定量显示了感染时间与病毒滴度之间的相关性。所有这些结果表明，我们已经通过反向遗传技术成功地从RK13细胞中回收了RHDV，这对于研究RHDV的抗原性，毒力，致病性，成熟性和新型疫苗非常有用。

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