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首页> 外文期刊>Chinese science bulletin >In situ AFM observation of BSA adsorption on HOPG with nanobubble
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In situ AFM observation of BSA adsorption on HOPG with nanobubble

机译：纳米气泡对BSA吸附BSA的原位原子力显微镜观察

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摘要

Nanobubbles have been proven existent at the liquid/solid interface, and become a focus of researchon varied interfacial processes. In the present work, by observing in situ with atomic force microscope(AFM), we found that nanobubbles could influence the adsorption process of bovine serum albumin(BSA) on hydrophobic surface of highly ordered pyrolytic graphite (HOPG). BSA could adsorb evenly,and coexist with nanobubbles at water/HOPG interface. After removing nanobubbles by injectingethanol, some hollows were found in the BSA layers at the same positions of nanobubbles existingpreviously. These hollows were about 8 nm in depth and dozens of nanometers in diameter. The correlation coefficient between the areas of nanobubbles and that of the corresponding hollows reached0.88--0.94, which strongly supported the assumption that the hollows were indeed caused by thenanobubbles. Moreover, the BSA molecules formed rings around the nanobubbles, suggesting thepreference of BSA adsorption onto the contact line at water/HOPG interface.
机译：纳米气泡已被证明存在于液体/固体界面，并成为各种界面过程研究的重点。在目前的工作中，通过原子力显微镜（AFM）的原位观察，我们发现纳米气泡可以影响牛血清白蛋白（BSA）在高度有序热解石墨（HOPG）疏水表面上的吸附过程。牛血清白蛋白可以均匀吸附，并与纳米气泡在水/ HOPG界面共存。通过注入乙醇去除纳米气泡后，在BSA层中先前存在的纳米气泡的相同位置发现了一些空洞。这些空洞的深度约为8 nm，直径为数十纳米。纳米气泡的面积与相应的空洞的面积之间的相关系数达到0.88--0.94，这强烈支持了空洞确实是由纳米气泡引起的这一假设。此外，BSA分子在纳米气泡周围形成环，表明BSA优先吸附在水/ HOPG界面的接触线上。

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