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SCFP, a novel fiber-specific promoter in cotton

机译：SCFP，棉花中一种新型的纤维特异性启动子

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摘要

To investigate the expression pattern of GhSCFP which was isolated from cotton fiber cDNA library, a 1006 bp upstream fragment of the gene was cloned by chromosome walking and fused to GUS and GFP respectively. Histochemical GUS and GFP fluorescence analysis revealed that the expression of the report genes driven by the promoter sequence was detectable only in outer layer cells during the seed development in the transgentic tobaccos. In transgenic cotton, strong GUS activity was observed in spherical protrusions on 0 dpa (days post anthesis) ovule surface, and in the 2-36 dpa fiber cells, while no GUS signals were detected in the root, leaves, stem, corolla, anther and stigma. Our data demonstrated that GhSCFP upstream sequence is a cotton fiber-specific promoter and this promoter will be useful in the molecular research on fiber cell development and in cotton fiber improvements by genetic modification.
机译：为了研究从棉纤维cDNA文库中分离的GhSCFP的表达模式，通过染色体行走克隆了该基因的1006 bp上游片段，并分别与GUS和GFP融合。组织化学GUS和GFP荧光分析表明，由启动子序列驱动的报告基因的表达仅在转基因烟草种子发育过程中可在外层细胞中检测到。在转基因棉花中，在0 dpa（花后天）胚珠表面和2-36 dpa纤维细胞中观察到了强烈的GUS活性，而在根，叶，茎，花冠和花药中未检测到GUS信号和污名。我们的数据表明，GhSCFP上游序列是棉纤维特异性启动子，该启动子将用于纤维细胞发育的分子研究以及通过遗传修饰改善棉纤维。

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