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首页> 外文期刊>Chinese science bulletin >beta(2)-Adrenoceptor affinity chromatography and its application in the screening of the active compounds from Semen Armeniacae Amarum
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beta(2)-Adrenoceptor affinity chromatography and its application in the screening of the active compounds from Semen Armeniacae Amarum

机译：β（2）-肾上腺素受体亲和层析及其在筛选阿米精中活性成分中的应用

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摘要

beta(2)-Adrenoceptor (beta(2)-AR) was purified from the rabbit lung tissue by sepharose-salbutamol affinity chromatographic column. To prepare the beta(2)-AR stationary phase, beta(2)-AR was evenly immobilized on the surface of macro-pore silica with a mild chemical coupling method through covalent bond. The retention properties of beta(2)-AR stationary phase were characterized by four ligands, salbutamol sulfate, noradrenaline bitartrate, adrenaline hydrochloride and propranolol hydrochloride, to establish the beta(2)-AR affinity chromatography. Then, the method was used to screen the active compounds from the total extracts of Semen Armeniacae Amarum. The results showed that beta(2)-AR on the surface of the stationary phase could keep its original bioactivity and selectivity. Amygdalin retained in the chromatographic column was proved to be the active compound of the total extracts of Semen Armeniacae Amarum. Compared with the existing chromatographic screening approaches, this method showed a good stability and high selectivity. The active compounds which could interact with beta(2)-AR in traditional Chinese medicine (TCM) could be screened efficiently by this method, providing a new way to screen the active compounds in complicated samples such as TCM.
机译：β-（2）-肾上腺素受体（β（2）-AR）通过琼脂糖-沙丁胺醇亲和色谱柱从兔肺组织中纯化。为了制备β（2）-AR固定相，通过温和的化学偶联方法通过共价键将β（2）-AR均匀地固定在大孔二氧化硅的表面上。 β（2）-AR固定相的保留特性由四个配体硫酸沙丁胺醇，酒石酸去甲肾上腺素，盐酸肾上腺素和盐酸普萘洛尔表征，以建立β（2）-AR亲和色谱。然后，该方法用于从from子总提取物中筛选出活性化合物。结果表明，固定相表面上的beta（2）-AR可以保持其原始生物活性和选择性。保留在色谱柱中的苦杏仁苷被证明是Arm仁总提取物的活性化合物。与现有的色谱筛选方法相比，该方法具有良好的稳定性和高选择性。通过这种方法可以有效地筛选可能与中药β（2）-AR相互作用的活性化合物，为筛选中药等复杂样品中的活性化合物提供了一种新途径。

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