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首页> 外文期刊>Chinese science bulletin >Construction of AFLP-based genetic linkage maps for the Chinese shrimp Fenneropaeneus chinensis
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Construction of AFLP-based genetic linkage maps for the Chinese shrimp Fenneropaeneus chinensis

机译：基于AFLP的中华对虾无脊椎对虾遗传连锁图谱的构建

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摘要

Fenneropaeneus chinensis is an important species in marine fishery resources and aquaculture in China. A genetic linkage map is essential for improving the efficiency of its breeding by marker-assisted selection and identifying commercially important genes. Linkage maps of F chinensis were constructed with an F2 mapping population (110 progenies) using amplified fragment length polymorphic (AFLP) marker in this study. Fifty-five AFLP primer combinations produced 532 AFLP markers fitting for map strategy in mapping family. The markers with 3:1 segregating ratios were analyzed using F2 intercross model for the common linkage map, while the markers with 1:1 ratio were analyzed using the pseudo-testcross strategy. The maps of male, female and common were constructed. The female map included 103 markers that formed 28 linkage groups, covering a total length of 1090 M All markers were linked with the linkage groups. Segregation distortion was observed for 6 of 103 markers in the female map. The average distance between markers was 14.53 cM and ranged from 4.4 to 24.8 M The male map included 144 markers that formed 35 linkage groups. Ten markers remained unlinked in male map. Segregation distortion was observed for 7 of 144 markers in the male map. The total distance of male map covered 1617 M The average distance between markers was 16.36 cM. The male map was 32.6% longer than the female map, which may reflect sex-specific recombination rates in Chinese shrimp. The common map was composed of 216 markers, including in 44 linkage groups covering a total distance of 1772.1 cM. Two markers remained unlinked. No distorted markers of 216 markers were shown in the common map. The distance between markers was 10.42 M An average estimated genome size for the Chinese shrimp was 2420 cM, which was consistent with the relative size of the Penaeid genome. The distribution of AFLP markers was relatively even in chromosomes of Chinese shrimp maps. The linkage analysis presented in this work provided some insight into the level of polymorphism and genetic variation of Chinese shrimp.
机译：中华蕨（Fenneropaeneus chinensis）是中国海洋渔业资源和水产养殖中的重要物种。遗传连锁图谱对于通过标记辅助选择和鉴定具有商业意义的基因来提高其育种效率至关重要。在这项研究中，使用扩增的片段长度多态性（AFLP）标记，利用F2作图种群（110个子代）构建了中华白对虾的连锁图谱。 55种AFLP引物组合产生了532个AFLP标记，适合作图家族中的作图策略。使用F2交叉模型对常见连锁图分析具有3：1隔离比的标记，而使用伪testcross策略分析具有1：1比例的标记。绘制了男性，女性和普通人的地图。雌性图谱包括103个标记，形成28个连锁组，全长1090M。所有标记均与连锁组相连。在雌性图谱中观察到103个标记中的6个的分离变形。标记之间的平均距离为14.53 cM，范围为4.4至24.8M。男性图包括144个标记，形成35个连锁基团。十个标记在男性图中仍然未链接。在雄性图谱中观察到144个标记中的7个的分离畸变。男性图谱的总距离为1617M。标记之间的平均距离为16.36 cM。雄性图比雌性图长32.6％，这可能反映了中国虾的性别特异性重组率。通用图谱由216个标记组成，包括44个连锁组，总距离为1772.1 cM。两个标记仍未关联。普通图中未显示216个标记的扭曲标记。标记之间的距离为10.42M。中国虾的平均估计基因组大小为2420 cM，这与对虾基因组的相对大小一致。 AFLP标记在中国虾图谱中的分布相对均匀。这项工作中提出的连锁分析为中国虾的多态性水平和遗传变异提供了一些见识。

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