Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy

Fan XiaoJun; Zheng Bo; Fu YueJun; Sun Yi; Liang AiHua

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Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy

机译：杆状病毒介导的蝎子神经毒素表达提高杀虫效力

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An Buthus martensii Karsch Insect Toxin (BmK IT) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1 x 10(7) PIBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6 h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8 h) during a 192 h infection.

机译：将Buthus martensii Karsch昆虫毒素（BmK IT）基因插入到加州产的Autographa californica多衣壳核多角体病毒（AcMNPV）基因组中，以构建重组杆状病毒AcMNPV-BmK IT。使用RT-PCR，斑点印迹和SDS-PAGE分析证实了BmK IT的表达。在野生型杆状病毒AcMNPV和重组病毒AcMNPV-BmK IT之间比较了对甜菜夜蛾幼虫的剂量-致死时间响应。在浓度为1 x 10（7）PIBs / mL的情况下，重组杆状病毒在第三龄幼虫S. exigua幼虫上的平均致死时间（LT50 = 73.6 h）比野生型病毒（LT50 =在感染192小时后的84.8小时）。

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《Chinese science bulletin》 |2008年第12期|共6页
作者
Fan XiaoJun; Zheng Bo; Fu YueJun; Sun Yi; Liang AiHua;
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正文语种 eng
中图分类自然科学现状及发展;
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dose-lethal time response;

机译：剂量致死时间反应;

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专利

1. Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy [J] . Fan XiaoJun, Zheng Bo, Fu YueJun, Chinese science bulletin . 2008,第12期

机译：杆状病毒介导的蝎子神经毒素表达提高杀虫效力
2. Regulation analysis of AcMNPV-mediated expression of a Chinese scorpion neurotoxin under the IE1, P10 and PH promoter in vivo and its use as a potential bio-insecticide [J] . Fu Yuejun, Li Xing, Du Jun, Biotechnology Letters . 2015,第10期

机译：体内IE1，P10和pH启动子下中国蝎子神经毒素的ACMNPV介导的调节分析及其用作潜在的生物杀虫剂
3. Baculovirus‐mediated expression of a scorpion depressant toxin improves the insecticidal efficacy achieved with excitatory toxins [J] . Chejanovsky Nor, Froy Oren, Gershburg Eduard, FEBS Letters . 1998,第2期

机译：杆状病毒介导的蝎子抑制剂毒素的表达提高了兴奋性毒素的杀虫功效
4. Fluorescence of native and partially denatured variant-3 scorpion neurotoxin [C] . Christopher Haydock, Salah S. Sedarous, Franklyn G. Prendergast Fluorescence Science and Technology . 2000

机译：天然和部分变性变异-3蝎神经毒素的荧光
5. Developing the next generation of chemical and bioinsecticides from insecticidal neurotoxins of Hadronyche versuta. [D] . Maggio, Francesco. 2004

机译：从Verdro Hadronyche的杀虫神经毒素开发下一代化学和生物杀虫剂。
6. Characterization of a membrane binding loop leads to engineering botulinum neurotoxin B with improved therapeutic efficacy [O] . Linxiang Yin, Geoffrey Masuyer, Sicai Zhang, 2020

机译：膜结合环的表征导致工程肉毒杆菌神经毒素B具有改善的治疗功效
7. Baculovirus-mediated expression of a scorpion depressant toxin improves the insecticidal efficacy achieved with excitatory toxins [O] . Gershburg Eduard, Stockholm Daniel, Froy Oren, 1998

机译：杆状病毒介导的蝎子抑制毒素表达提高了兴奋性毒素的杀虫功效
8. Precursors of Androctonus australis Scorpion Neurotoxins. Structures of Precursors, Processing Outcomes, and Expression of a Functional Recombinant Toxin II [R] . Bougis, P. E., Rochat, H., Smith, L. A. 1989

机译：androctonus australis scorpion Neurotoxins的前体。前体结构，加工结果和功能性重组毒素II的表达

Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy

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