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首页> 外文期刊>Yeast >Identification of a Candida glabrata homologue of the S-cerevisiae VRG4 gene, encoding the Golgi GDP-mannose transporter
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Identification of a Candida glabrata homologue of the S-cerevisiae VRG4 gene, encoding the Golgi GDP-mannose transporter

机译：鉴定S-啤酒酵母VRG4基因的光滑念珠菌同源物，该基因编码高尔基GDP-甘露糖转运蛋白

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摘要

Mannoproteins on the cell wall of yeast and fungi help regulate cell shape, porosity, and cell-cell interactions, including those required for attachment to host cells by fungal pathogens. The mannose-containing oligosaccharides on proteins and lipids are extended in the Golgi by glycosyltransferases that use GDP-mannose as the sugar substrate. A membrane-bound transporter that, in Saccharomyces cerevisiae, is encoded by the VRG4 gene catalyses delivery of GDP-mannose into the lumen of the Golgi. We report here the cloning of the homologous VRG4 gene from the pathogenic yeast, Candida glabrata, by functional complementation of an S. cerevisiae vrg4 mutant. The sequence of the CgVrg4 protein displays significant homology to GDP-mannose transporters from other yeast, fungi, protozoa, and plants. CgVRG4 fully complements the glycosylation defect and other cell wall associated vrg4 mutant phenotypes. Like ScVRG4, CgVRG4 is essential for the viability of C. glabrata. These results suggest that, as in S. cerevisiae, CgVrg4p accounts for all of the GDP-mannose transport activity in the Golgi lumen. The GenBank Accession No. for CgVRG4 is #AF360395. Copyright
机译：酵母和真菌细胞壁上的甘露糖蛋白有助于调节细胞的形状，孔隙度和细胞间的相互作用，包括真菌病原体附着在宿主细胞上所需的那些。通过使用GDP-甘露糖作为糖底物的糖基转移酶，蛋白质和脂质上的含甘露糖寡糖在高尔基体中延伸。在酿酒酵母中，由VRG4基因编码的膜结合转运蛋白可催化将GDP-甘露糖输送到高尔基体腔中。我们在这里报告通过功能互补的酿酒酵母vrg4突变体从致病酵母，光滑念珠菌克隆同源VRG4基因。 CgVrg4蛋白的序列与来自其他酵母，真菌，原生动物和植物的GDP-甘露糖转运蛋白显示出显着的同源性。 CgVRG4完全补充了糖基化缺陷和其他与细胞壁相关的vrg4突变表型。像ScVRG4一样，CgVRG4对于光滑念珠菌的生存至关重要。这些结果表明，与酿酒酵母一样，CgVrg4p占据了高尔基体腔中所有GDP-甘露糖运输活动。 CgVRG4的GenBank登录号为＃AF360395。版权

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