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首页> 外文期刊>Yeast >Molecular cloning and DNA analysis of a gene encoding alpha mating pheromone from the yeast Saccharomyces naganishii
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Molecular cloning and DNA analysis of a gene encoding alpha mating pheromone from the yeast Saccharomyces naganishii

机译：Naganishii酵母中编码α交配信息素的基因的分子克隆和DNA分析

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A DNA fragment encoding the precursor peptide for a mating pheromone was isolated from the S. naganishii genome based on the amino acid sequence of the mature pheromone. The precursor peptide contains three copies of the pheromone. Hydrophobicity analysis of the precursor peptide revealed an N-terminal signal sequence for translocation into the lumen of the endoplasmic reticulum and several signals for a series of secretion-related processes. However, upstream regulatory sequences necessary for expression of the S. cerevisiae alpha mating pheromone gene were not found, suggesting the divergence of systems that regulate a mating pheromone gene expression in S. naganishii and S. cerevisiae. Hybridization of a probe corresponding to the S. naganishii a mating pheromone nucleotide sequence to S. naganishii chromosomal DNA revealed a single gene located on either chromosome VI or VII. The S. naganishii a mating pheromone sequence has been deposited in the DDBJ/EMBL/GenBank data library under Accession No. AB086431.
机译：基于成熟信息素的氨基酸序列，从长链志贺菌基因组中分离出编码交配信息素的前体肽的DNA片段。前体肽包含三份信息素。前体肽的疏水性分析显示了一个N端信号序列，可转位进入内质网腔，以及一些与一系列分泌相关过程有关的信号。但是，未找到表达酿酒酵母α交配信息素基因所必需的上游调控序列，这表明调节长链酵母和酿酒酵母中交配信息素基因表达的系统的差异。与交配的信息素核苷酸序列的长根链球菌对应的探针与长根链球菌染色体DNA的杂交揭示了位于染色体VI或VII上的单个基因。 naganishii交配信息素序列已保藏在DDBJ / EMBL / GenBank数据库中，登录号为AB086431。

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