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首页> 外文期刊>Yeast >Comparative proteomic analysis of a Candida albicans DSE1 mutant under filamentous and non-filamentous conditions
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Comparative proteomic analysis of a Candida albicans DSE1 mutant under filamentous and non-filamentous conditions

机译：丝状和非丝状条件下白色念珠菌DSE1突变体的比较蛋白质组学分析

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摘要

Candida albicans is a common opportunistic pathogen that causes a variety of diseases in immunocompromised hosts. In a pathogen, cell wall proteins are important virulence factors. We previously characterized Dse1 as a cell wall protein necessary for virulence and resistance to cell surface-disrupting agents, such as Calcofluor white, chitin deposition, proper adhesion and biofilm formation. In the absence of decomplexation, our objectives were to investigate differential proteomic expression of a DSE1 mutant strain compared to the wild-type strain. The strains were grown under filamentous and non-filamentous conditions. The extracted cell proteome was subjected to tryptic digest, followed by generation of peptide profiles using MALDI-TOF MS. Generated peptide profiles were analysed and unique peaks for each strain and growth condition mined against a Candida database, allowing protein identification. The DSE1 mutant was shown to lack the chitin biosynthesis protein Chs5, explaining the previously observed decrease in chitin biosynthesis. The wild-type strain expressed Pra1, involved in pH response and zinc acquisition, Atg15, a lipase involved in virulence, and Sod1, required for oxidative stress tolerance, in addition to proteins involved in protein biosynthesis, explaining the increase in total protein content observed compared to the mutants strain. The mutant, on the other hand, expressed glucoamylase 1, a cell wall glycoprotein involved in carbohydrate metabolism cell wall degradation and biofilm formation. As such, MALDI-TOF MS is a reliable technique in identifying mutant-specific protein expression in C. albicans. Copyright (c) 2014 John Wiley & Sons, Ltd.
机译：白色念珠菌是一种常见的机会病原体，可在免疫受损的宿主中引起多种疾病。在病原体中，细胞壁蛋白是重要的毒力因子。我们以前将Dse1表征为细胞壁蛋白，该蛋白对于毒力和对细胞表面破坏剂（如钙荧光白，几丁质沉积，适当的粘附和生物膜形成）的抵抗力是必需的。在没有解复合的情况下，我们的目标是研究与野生型菌株相比，DSE1突变菌株的差异蛋白质组表达。菌株在丝状和非丝状条件下生长。对提取的细胞蛋白质组进行胰蛋白酶消化，然后使用MALDI-TOF MS生成肽谱。分析了产生的肽谱，并根据假丝酵母数据库提取了每种菌株和生长条件的独特峰，从而可以鉴定蛋白质。 DSE1突变体显示缺乏几丁质生物合成蛋白Chs5，解释了先前观察到的几丁质生物合成减少。除了参与蛋白质生物合成的蛋白质之外，野生型菌株还表达了参与pH响应和锌获取的Pra1，参与毒力的脂肪酶Atg15和氧化应激所需的Sod1，这解释了参与蛋白质生物合成的蛋白质，这说明观察到的总蛋白质含量增加与突变株相比。另一方面，该突变体表达葡糖淀粉酶1，一种参与糖代谢细胞壁降解和生物膜形成的细胞壁糖蛋白。因此，MALDI-TOF MS是鉴定白色念珠菌中突变体特异性蛋白表达的可靠技术。版权所有（c）2014 John Wiley＆Sons，Ltd.

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