...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Yeast >Revisiting the yeast chromosome VI DNA sequence reveals a correction merging YFL007w and YFL006w to a single ORF
【24h】

Revisiting the yeast chromosome VI DNA sequence reveals a correction merging YFL007w and YFL006w to a single ORF

机译：重新审查酵母染色体VI DNA序列揭示了将YFL007w和YFL006w合并为单个ORF的校正

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The overall contiguity of the Saccharomyces cerevisiae chromosome VI sequence assembly was assessed by systematic long-range PCR, PCR product size determination and sequencing. Using S. cerevisiae strain FY1679 total genomic DNA as template, 41 overlapping PCR products were generated, covering the complete 270 kb chromosome VI sequence. Specificity of the PCR products was confirmed by direct end-sequencing. No fragment size discrepancies with the published sequence were observed, confirming the overall sequence assembly. Gel reads of the PCR-fragment ends compile to a total of resequenced DNA representing 16% of the entire chromosome VI and reveal three single nucleotide differences. One of these is an extra G in the protein-coding region of YFL007w. Due to this additional nucleotide, the coding sequences of YFL007w, and YFL006w become part of a 6432 bp ORF. The same sequence also resulted from analysis of strain BY4743. Homologous proteins of unknown function found in Candida albicans, Arabidopsis thaliana, Caenorhabditis elegans and man, as well as comparative data from published transcript profiles of YFL006w and YFL007w, give additional evidence for the existence of a single gene at this locus in yeast. Copyright
机译：通过系统的长距离PCR，PCR产物大小的确定和测序来评估酿酒酵母染色体VI序列装配体的总体连续性。使用酿酒酵母菌株FY1679总基因组DNA作为模板，生成了41个重叠的PCR产物，覆盖了完整的270 kb染色体VI序列。通过直接末端测序证实了PCR产物的特异性。没有观察到与公开序列的片段大小差异，证实了整个序列组装。 PCR片段末端的凝胶读数被编译为代表整个VI染色体16％的总重测序DNA，并揭示了三个单核苷酸差异。其中之一是YFL007w蛋白质编码区中的额外G。由于该额外的核苷酸，YFL007w和YFL006w的编码序列成为6432 bp ORF的一部分。相同序列也来自菌株BY4743的分析。在白色念珠菌，拟南芥，秀丽隐杆线虫和人中发现的功能未知的同源蛋白质，以及来自YFL006w和YFL007w的已发表转录本概况的比较数据，为酵母中该基因座上存在单个基因提供了更多证据。版权

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号