Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non-artificial conditions

Elke Nevoigt; Anne Fassbender; Ulf Stahi

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Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non-artificial conditions

机译：酵母酿酒酵母的细胞可在非人工条件下通过DNA转化

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Transformants of bakers' yeast (Saccharomyces cerevisiae) can be generated when nongrowing cells metabolize sugars (without additional nutrients) in the presence of plaid DNA. These results suggest that there is a mechanism by which DNA can naturally be taken up by the yeast cell. Natural transformation does not take place in common complete or minimal yeast culture media such as YPD and YNB. The starvation conditions used in our experiments thus seem to be an important prerequisite for such transformation events.

机译：当非生长细胞在有格子DNA的情况下代谢糖（无其他营养素）时，可以产生面包酵母（啤酒酵母）的转化体。这些结果表明存在一种机制，通过该机制DNA可以自然地被酵母细胞吸收。在常见的完全或基本酵母培养基（例如YPD和YNB）中不会发生自然转化。因此，我们的实验中使用的饥饿条件似乎是发生此类转化事件的重要前提。

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《Yeast》 |2000年第12期|共4页
作者
Elke Nevoigt; Anne Fassbender; Ulf Stahi;
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原文格式 PDF
正文语种 eng
中图分类酿造工业;
关键词
Saccharomyces cerevisiae; fermentation; natural transformation; horizontal gene transfer;

机译：酿酒酵母;发酵;自然转化;水平基因转移;

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1. Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non-artificial conditions [J] . Elke Nevoigt, Anne Fassbender, Ulf Stahi Yeast . 2000,第12期

机译：酵母酿酒酵母的细胞可在非人工条件下通过DNA转化
2. Heterologous Expression of the Carrot Hsp17.7 gene Increased Growth, Cell Viability, and Protein Solubility in Transformed Yeast (Saccharomyces cerevisiae) under Heat, Cold, Acid, and Osmotic Stress Conditions [J] . Ko Eunhye, Kim Minhye, Park Yunho, Journal of Apicultural Research . 2016,第3期

机译：红萝卜HSP17.7基因的异源表达增加了在热，冷，酸和渗透压条件下转化的酵母（酿酒酵母）中的生长，细胞活力和蛋白质溶解度
3. Running on empty: Does mitochondrial DNA mutation limit replicative lifespan in yeast? Mutations that increase the division rate of cells lacking mitochondrial DNA also extend replicative lifespan in Saccharomyces cerevisiae [J] . Dunn Cory D. . BioEssays : . 2011,第10期

机译：空空运行：线粒体DNA突变是否会限制酵母中的复制寿命？增加缺乏线粒体DNA的细胞分裂率的突变也延长了酿酒酵母的复制寿命
4. Machine learning approach to segment Saccharomyces cerevisiae yeast cells [C] . Tleis Mohamed, Verbeek Fons International Conference on Advances in Biomedical Engineering . 2015

机译：机器学习方法来分割酿酒酵母酵母细胞
5. Artificial cell -cell communication and multi-cellular pattern formation in yeast Saccharomyces cerevisiae [D] . Chen, Ming-Tang 2008

机译：酿酒酵母中的人工细胞间通讯和多细胞模式形成
6. Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences. [O] . R H Schiestl, M Dominska, T D Petes 1993

机译：用非同源DNA转化啤酒酵母：将转化DNA非法整合到酵母染色体中并将转化DNA体内结合到线粒体DNA序列中。
7. Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences. [O] . Schiestl, R H, Dominska, M, Petes, T D 1993

机译：用非同源DNA转化啤酒酵母：将转化DNA非法整合到酵母染色体中，并将转化DNA体内结合到线粒体DNA序列中。

Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non-artificial conditions

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