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Rapid and reliable protein extraction from yeast

机译:从酵母中快速可靠地提取蛋白质

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摘要

The methods currently used for protein extraction from yeast are either laborious or insufficiently reliable. Here I report a method for protein extraction for electrophoretic analysis that is both easy and reliable. In this method, yeast cells are subjected to mild alkali treatment and then boiled in a standard electrophoresis loading buffer. The method was tested for different strains of Saccharomyces ceverisiae and for yeast Hansenula polymorpha DL-I. It yields virtually complete extraction independently of the strain, growth conditions and protein molecular weight and allows working with small amounts of yeast cells grown on agar plates. Copyright (C) 2000 John Wiley & Sons, Ltd.
机译:当前用于从酵母提取蛋白的方法费力或可靠性不足。在这里,我报告了一种用于电泳分析的蛋白质提取方法,该方法既简单又可靠。在这种方法中,酵母细胞经过温和的碱处理,然后在标准的电泳上样缓冲液中煮沸。测试了该方法用于酿酒酵母的不同菌株和酵母多形汉逊酵母DL-1的能力。它几乎可以完全提取出来,而与菌株,生长条件和蛋白质分子量无关,并且可以与在琼脂平板上生长的少量酵母细胞一起工作。版权所有(C)2000 John Wiley&Sons,Ltd.

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