• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chinese science bulletin >Cloning, sequencing and expression analysis of cDNA encoding a constitutive heat shock protein 70 (HSC70) in Fenneropenaeus chinensis
【24h】

Cloning, sequencing and expression analysis of cDNA encoding a constitutive heat shock protein 70 (HSC70) in Fenneropenaeus chinensis

机译：费氏对虾热休克蛋白70（HSC70）的cDNA的克隆，序列分析及表达分析

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The cDNA encoding hsc70 of Chinese shrimp Fenneropenaeus chinensis was cloned from hepatopancreas by RT-PCR based on its EST sequence. The full length cDNA of 2090 bp contained an open reading frame of 1956 nucleotides and partial 5'- and 3'-untranslated region(5'- and 3'-UTR). PCR amplification and sequencing analysis showed the existence of introns fa the region of 1-547 bp, but they did not exist in the region of 548-2090 bp of hsc70 cBNA. When the deduced 652 amino acid sequence of HSC70 was compared with the members of HSP70 family from other organisms, the results showed 85.9 percent similarity with HSC71 from Oncorhynchus mykiss and HSC70 from Homo sapiens. It also exhibited 85.8 percent similarity with HSP70 from Mus inusculu and 85.4 percent with HSC70 from Manduca sexta. Expression analysis showed that hsc70 mRNA was espressed constitutively in hepatopancreas, muscle, eyestalks, haemocytes, heart, ovary, intestine and gills in Fenneropenaeus chinensis. No difference could be detected on hsc70 mRNA level in muscle between heat-shocked and control animals.
机译：根据其胰腺EST序列，通过RT-PCR从肝胰腺中克隆了中国虾对虾hsc70的cDNA编码。 2090 bp的全长cDNA包含一个1956个核苷酸的开放阅读框以及部分5'-和3'-非翻译区（5'-和3'-UTR）。 PCR扩增和测序分析表明，在hsc70 cBNA的1-4547 bp区域存在内含子，但在548-2090 bp区域不存在内含子。将推导的HSC70的652个氨基酸序列与其他生物的HSP70家族的成员进行比较时，结果显示与Oncorhynchus mykiss的HSC71和智人的HSC70有85.9％的相似性。它也表现出与Mus inusculu的HSP70的相似度为85.8％，与Manduca sexta的HSC70的相似度为85.4％。表达分析表明，hsc70 mRNA在中国猪鞭草的肝胰腺，肌肉，眼球，血细胞，心脏，卵巢，肠和and中组成性表达。在热休克动物和对照动物之间，肌肉中的hsc70 mRNA水平没有差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号