Interbilayer lipid mixing induced by the human immunodeficiency virus type-1 fusion peptide on large unilamellar vesicles: the nature of the nonlamellar intermediates.

Pereira FB; Basanez G; Goni FM; Nieva JL; Valpuesta JM

首页> 外文期刊>Chemistry and Physics of Lipids >Interbilayer lipid mixing induced by the human immunodeficiency virus type-1 fusion peptide on large unilamellar vesicles: the nature of the nonlamellar intermediates.

【24h】

Interbilayer lipid mixing induced by the human immunodeficiency virus type-1 fusion peptide on large unilamellar vesicles: the nature of the nonlamellar intermediates.

机译：人免疫缺陷病毒1型融合肽在大单层囊泡上诱导的双层间脂质混合：非薄片中间体的性质。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A peptide corresponding to the 23 N-terminal amino acid residues of the human immunodeficiency virus type-1 (HIV-1) gp41 has the capacity to induce intervesicular lipid mixing in large unilamellar liposomes composed of dioleoylphosphatidylcholine (DOPC), dioleoylphosphatidylethanolamine (DOPE) and cholesterol (CHOL) (molar ratio, 1:1:1). Cryo-transmission electron microscopy (cryo-TEM) of diluted vesicles to which peptides has been externally added reveals a morphology that is compatible with the formation of nonlamellar lipidic aggregates during the time-course of lipid mixing. 31P-nuclear magnetic resonance and 1-(4-trimethylaminophenyl)-6-phenyl-1,3,5-hexatriene (TMADPH) steady-state anisotropy data at equilibrium indicate that the peptide is able to modulate the lipid polymorphism in pelletted membranes by: (i) promoting the thermotropic formation of inverted phases; and (ii) driving the lamellar-to-nonlamellar transition towards the formation of isotropic phases. Therefore, our combined morphological and spectroscopic data reveal the existence of a direct correlation between the ability of the externally added peptide to induce lipid-mixing in dilute liposome samples and its capacity to modulate lipid polymorphism in stacked bilayers.

机译：对应于1型人类免疫缺陷病毒（HIV-1）gp41的23个N末端氨基酸残基的肽具有诱导大单层脂质体中囊泡脂质混合的能力，这些脂质体由二油酰基磷脂酰胆碱（DOPC），二油酰基磷脂酰乙醇胺（DOPE）和胆固醇（CHOL）（摩尔比为1：1：1）。从外部添加了肽的稀释囊泡的低温透射电子显微镜（cryo-TEM）揭示了一种与脂质混合过程中非薄片脂质聚集体形成相容的形态。平衡状态下的31P核磁共振和1-（4-三甲基氨基苯基）-6-苯基-1,3,5-己三烯（TMADPH）稳态各向异性数据表明，该肽能够通过以下方式调节沉淀膜中的脂质多态性：（i）促进反相的热致性形成; （ii）推动层状到非层状过渡向各向同性相的形成。因此，我们组合的形态学和光谱数据揭示了外部添加的肽在稀释的脂质体样品中诱导脂质混合的能力与其调节堆叠双层中脂质多态性的能力之间存在直接关系。

著录项

来源
《Chemistry and Physics of Lipids》 |1999年第2期|共10页
作者
Pereira FB; Basanez G; Goni FM; Nieva JL; Valpuesta JM;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
Amino Acid Sequence; Cholesterol: Chemistry; Cryoelectron Microscopy; Human; HIV Envelope Protein gp41: Chemistry; HIV Envelope Protein gp41: Ultrastructure; HIV-1; Kinetics; Lipid Bilayers: Chemistry; Molecular Sequence Data; Peptide Fragments: Chemistry; Peptide Fragments: Ultrastructure; Phosphatidylcholines: Chemistry; Phosphatidylethanolamines: Chemistry;

机译：氨基酸序列;胆固醇：化学;冷冻电子显微镜;人类;HIV包膜蛋白gp41：化学;HIV包膜蛋白gp41：超微结构;HIV-1;运动学;脂质双层：化学;分子序列数据;肽片段：化学;肽片段：超微结构;磷脂酰胆碱：化学;磷脂酰乙醇胺：化学;

相似文献

外文文献
专利

1. Interbilayer lipid mixing induced by the human immunodeficiency virus type-1 fusion peptide on large unilamellar vesicles: the nature of the nonlamellar intermediates. [J] . Pereira FB, Basanez G, Goni FM, Chemistry and Physics of Lipids . 1999,第1a2期

机译：人免疫缺陷病毒1型融合肽在大单层囊泡上诱导的双层间脂质混合：非薄片中间体的性质。
2. LYSIS OF LARGE UNILAMELLAR VESICLES INDUCED BY ANALOGS OF THE FUSION PEPTIDE OF INFLUENZA VIRUS HEMAGGLUTININ [J] . Soltesz SA., Hammer DA. Journal of Colloid and Interface Science . 1997,第2期

机译：流感病毒血凝素融合肽类似物诱导的大单层细胞裂解
3. Preparing giant unilamellar vesicles (GUVs) of complex lipid mixtures on demand: Mixing small unilamellar vesicles of compositionally heterogeneous mixtures [J] . Bhatia Tripta, Husen Peter, Brewer Jonathan, Biochimica et biophysica acta. Biomembranes . 2015,第12期

机译：按需准备复杂脂质混合物的巨型单层囊泡（GUV）：混合组成不同的混合物的小的单层囊泡
4. Endocytosi of Giant Unilamellar Vesicles as Induced by Bola Peptide [C] . Qiuhong Yu, Dehai Liang The 12th International Symposium on Polymer Physics(PP’2016)(2016年第十二届国际高分子物理学术讨论会）论文集 . 2016

机译：Bola肽诱导的巨单层囊泡的胞吞
5. Epitope-targeting strategy for a vaccine against human immunodeficiency virus type-1: Peptide ligands for the broadly-neutralizing antibodies b12, 2F5 and 2G12. [D] . Menendez, Alfredo. 2005

机译：针对1型人类免疫缺陷病毒的疫苗的表位靶向策略：广泛中和的抗体b12、2F5和2G12的肽配体。
6. pH-Dependent Vesicle Fusion Induced by the Ectodomain of the Human Immunodeficiency Virus Membrane Fusion Protein gp41: Two Kinetically Distinct Processes and Fully-Membrane-Associated gp41 with Predominant β Sheet Fusion Peptide Conformation [O] . Punsisi U. Ratnayake, Kelly Sackett, Matthew J. Nethercott, -1

机译：由人类免疫缺陷病毒膜融合蛋白gp41的Ecdomain诱导的pH依赖性囊泡融合：两个动力学上不同的过程和与膜相关的gp41与完整的β折叠融合肽构型。
7. pH-dependent vesicle fusion induced by the ectodomain of the human immunodeficiency virus membrane fusion protein gp41: Two kinetically distinct processes and fully-membrane-associated gp41 with predominant β sheet fusion peptide conformation [O] . Ratnayake Punsisi U., Sackett Kelly, Nethercott Matthew J., 2015

机译：人类免疫缺陷病毒膜融合蛋白gp41胞外域诱导的pH依赖性囊泡融合：两个动力学上不同的过程以及具有主要β折叠融合肽构象的全膜相关gp41

Interbilayer lipid mixing induced by the human immunodeficiency virus type-1 fusion peptide on large unilamellar vesicles: the nature of the nonlamellar intermediates.

摘要

著录项

相似文献

相关主题

期刊订阅