• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Histopathology: Official Journal of the British Division of the International Academy of Pathology >Comparative evaluation of nm23 and p16 expression as biomarkers of high-risk human papillomavirus infection and cervical intraepithelial neoplasia 2(+) lesions of the uterine cervix.
【24h】

Comparative evaluation of nm23 and p16 expression as biomarkers of high-risk human papillomavirus infection and cervical intraepithelial neoplasia 2(+) lesions of the uterine cervix.

机译：nm23和p16表达作为高危型人乳头瘤病毒感染和子宫颈宫颈上皮内瘤样变2（+）病变的生物标志物的比较评估。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIMS: To investigate the clinical role of nm23 expression in identifying both high-risk human papillomavirus (HR-HPV) and high-grade cervical lesions or carcinomas [cervical intraepithelial neoplasia 2(+) (CIN2(+) )], and to compare it with p16 overexpression, as this latter biomarker has already been reported widely in HR-HPV infected cervical lesions. METHODS AND RESULTS: Immunohistochemical evaluation of nm23 and p16 in 143 cervical biopsy specimens including negative, low- and high-grade lesions and squamous carcinomas (SC). HR-HPV testing by Digene hybrid capture 2 (HC2) and polymerase chain reaction (PCR) on the cervico-vaginal samples of the same patients. In detecting CIN2(+) , p16 was significantly more sensitive and specific than nm23 (96.3% versus 81.8% and 66% versus 36.4%, respectively, both P < 0.0001). Concerning HR-HPV detection by HC2, p16 showed a significantly higher specificity than nm23 (82% versus 47%, P <0.0001), although the sensitivities were comparable (71% versus 76%). We found a significantly direct correlation between nm23 and HC2 findings. However, nm23 expression did not correlate with HPV16/18 infection. In contrast, we observed a significant association between p16 overexpression and HPV16/18 genotypes. CONCLUSIONS: We confirm the diagnostic value of p16 overexpression. Moreover, despite in vitro data regarding the interaction with the HPV-E7 protein, nm23 does not appear to be a more useful biomarker than p16 in identifying CIN2(+) or HR-HPV infection.
机译：目的：探讨nm23表达在鉴定高危型人乳头瘤病毒（HR-HPV）和高级别宫颈病变或癌[宫颈上皮内瘤变2（+）（CIN2（+））]中的临床作用，并进行比较它具有p16过度表达，因为后者已被广泛报道在HR-HPV感染的宫颈病变中。方法和结果：143份宫颈活检标本中nm23和p16的免疫组织化学评估，包括阴性，低度和高度病变以及鳞癌（SC）。通过Digene Hybrid Capture 2（HC2）和聚合酶链反应（PCR）对同一患者的宫颈阴道样品进行HR-HPV检测。在检测CIN2（+）时，p16的灵敏度和特异性比nm23高得多（分别为96.3％对81.8％和66％对36.4％，两者均P <0.0001）。关于通过HC2检测HR-HPV，p16的特异性明显高于nm23（82％对47％，P <0.0001），尽管敏感性相当（71％对76％）。我们发现nm23和HC2发现之间存在明显的直接相关性。但是，nm23表达与HPV16 / 18感染无关。相反，我们观察到p16过表达与HPV16 / 18基因型之间存在显着关联。结论：我们证实了p16过表达的诊断价值。而且，尽管有关于与HPV-E7蛋白相互作用的体外数据，nm23在鉴定CIN2（+）或HR-HPV感染方面似乎没有比p16更有用的生物标记。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号