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首页> 外文期刊>Histopathology: Official Journal of the British Division of the International Academy of Pathology >Whole-specimen histopathology: a method to produce whole-mount breast serial sections for 3-D digital histopathology imaging.
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Whole-specimen histopathology: a method to produce whole-mount breast serial sections for 3-D digital histopathology imaging.

机译：全标本组织病理学：一种产生用于3D数字组织病理学成像的整个乳腺连续切片的方法。

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摘要

AIMS: To develop a method for preparing diagnostic-quality, whole-mount serial sections of breast specimens while preserving 3-D conformation. This required supporting the fresh specimen prior to breadloafing and refining the conventional tissue processing method. The overall goal is to use digital images of whole-specimen histopathology to improve the estimation of extent of disease. METHODS AND RESULTS: To maintain a 3-D conformation, the specimen is suspended in 3.5% agar at 55 degrees C. The block is sliced at 5-mm intervals. Sectioning is performed after extended fixation in 4% formaldehyde from paraformaldehyde in 0.1 m Millonig's buffer, followed by paraffin processing using a non-routine schedule and extended paraffin infiltration. Whole-mount serial breast sections are produced with features of equal or superior quality to that which can be achieved using conventional methods. The method is compatible with some immunohistochemical stains but requires further optimization for others. CONCLUSIONS: The technique is currently suitable for research applications. With the reduction in processing time achievable with microwave-assisted processing, there is the potential for its use as a routine clinical method. This tool may improve the accuracy of margin estimates and identification of multifocality in breast cancer; further evaluation is necessary.
机译：目的：开发一种在保留3-D构象的同时准备诊断质量好的乳腺样品全系列系列切片的方法。这就需要在面包进行面包准备和精制常规组织处理方法之前支撑新鲜样本。总体目标是使用全标本组织病理学的数字图像来改善疾病范围的估计。方法和结果：为了保持3-D构象，将标本悬浮在55摄氏度的3.5％琼脂中。以5毫米的间隔切片。在0.1 m Millonig's缓冲液中将低聚甲醛中的4％甲醛中固定固定后，进行切片，然后使用非常规程序进行石蜡加工，并延长石蜡浸润度。整个系列的连续乳房切片所生产的产品具有与传统方法相同或更高的质量。该方法与某些免疫组织化学染色剂兼容，但需要对其他染色剂进一步优化。结论：该技术目前适合研究应用。随着微波辅助处理可缩短处理时间，其有可能用作常规临床方法。该工具可以提高利润率估计的准确性和乳腺癌多灶性的识别;有必要进行进一步评估。

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