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A new species of Pristimantis (Anura: Strabomantidae) from the pacific coast of the darien province, Panama, with a molecular analysis of its phylogenetic position

机译:来自达里安省太平洋海岸的一种新的Pristimantis(Anura:Strabomantidae)物种,通过分子进化分析

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摘要

We describe a new species of Pristimantis (Anura: Strabomantidae) from the Pacific coast of the Darién Province, Panama. The type locality is on Cerro Pi?a, in the Serranía de Sapo, at 700-800 m elevation. This new species is readily distinguished from all other known congeners from the region based on external morphology. Despite the small size of the two type specimens, histological analysis of the gonads shows these individuals were potentially reproductive males. Molecular phylogenetic analyses based on the ND2-WANCY mitochondrial gene region reveal that this new species is genetically distinct. Molecular and morphological data place the new species in the Pristimantis (Hypodictyon) ridens species series, and suggest that P. cerasinus be moved to this group as well. Molecular analyses also reveal a potential synapomorphy of the genus Pristimantis relative to the other five genera sampled here: the loss of the D-stem of the tRNA~(CYS) gene. Our finding of another endemic frog from eastern Panama lends further support to the concept of the Darién as a center of endemism and not just a conduit between continents.
机译:我们描述了来自巴拿马达里恩省太平洋海岸的一种新的Pristimantis(Anura:Strabomantidae)种。类型区域位于塞拉尼亚德萨波(Serraníade Sapo)的塞罗皮亚(Cerro Pi?a),海拔700-800 m。基于外部形态,该新物种很容易与该地区的所有其他已知同类物区分开。尽管这两种类型的标本很小,但对性腺的组织学分析表明,这些个体是潜在的生殖雄性。基于ND2-WANCY线粒体基因区域的分子系统发育分析表明,该新物种在遗传上是不同的。分子和形态学数据将新物种列为Pristimantis(Hypodictyon)骑乘物种系列,并建议将cerasinus cerasinus也移到这一类中。分子分析还揭示了相对于此处采样的其他五个属,Pristimantis属的潜在同形亚型:tRNA〜(CYS)基因的D-茎的丢失。我们从巴拿马东部发现的另一只地方性青蛙的发现,进一步支持了达里恩(Darién)概念作为地方性中心,而不仅仅是大陆之间的管道。

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