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首页> 外文期刊>Histochemistry and cell biology >Cellular expression of murine Ym1 and Ym2, chitinase family proteins, as revealed by in situ hybridization and immunohistochemistry.
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Cellular expression of murine Ym1 and Ym2, chitinase family proteins, as revealed by in situ hybridization and immunohistochemistry.

机译：原位杂交和免疫组化显示鼠Ym1和Ym2，几丁质酶家族蛋白的细胞表达。

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摘要

Ym is one of the chitinase family proteins, which are widely distributed in mammalian bodies and can bind glycosaminoglycans such as heparin/heparan sulfate. Ym1 is a macrophage protein produced in parasitic infections, while its isoform, Ym2, is upregulated in lung under allergic conditions. In the present study, we revealed the distinct cellular expression of Ym1 and Ym2 in normal mice by in situ hybridization and immunohistochemistry. Ym1 was principally expressed in the lung, spleen, and bone marrow, while Ym2 was found in the stomach. Ym1-expressing cells in the lung were alveolar macrophages, and the immunoreactivity for Ym1 was localized in rough endoplasmic reticulum. In the spleen, Ym1-expressing cells gathered in the red pulp and were electron microscopically identified as immature neutrophils. In the bone marrow, immature neutrophils were intensely immunoreactive, but lost this immunoreactivity with maturation. Moreover, needle-shaped crystals in the cytoplasm of macrophages, which formed erythroblastic islands, also showed intense Ym1 immunoreactivity. Ym2 expression was restricted to the stratified squamous epithelium in the junctional region between forestomach and glandular stomach. The function of Ym1 and Ym2 is still unclear; however, the distinct cellular localization under normal conditions suggests their important roles in hematopoiesis, tissue remodeling, or immune responses as an endogenous lectin.
机译：Ym是几丁质酶家族蛋白之一，其广泛分布在哺乳动物体内并且可以结合糖胺聚糖，例如肝素/硫酸乙酰肝素。 Ym1是一种在寄生虫感染中产生的巨噬细胞蛋白，而其亚型Ym2在变态反应条件下在肺中被上调。在本研究中，我们通过原位杂交和免疫组织化学揭示了正常小鼠中Ym1和Ym2的细胞表达。 Ym1主要在肺，脾和骨髓中表达，而Ym2在胃中发现。肺中表达Ym1的细胞是肺泡巨噬细胞，并且Ym1的免疫反应性位于粗糙的内质网中。在脾脏中，表达Ym1的细胞聚集在红髓中，并在电子显微镜下鉴定为未成熟的中性粒细胞。在骨髓中，未成熟的中性粒细胞具有强烈的免疫反应性，但随着成熟而丧失这种免疫反应性。此外，巨噬细胞的细胞质中形成针状增生小岛的针状晶体也表现出强烈的Ym1免疫反应性。 Ym2表达仅限于前胃与胃腺之间的交界区域中的分层鳞状上皮细胞。 Ym1和Ym2的功能仍然不清楚;然而，正常条件下的独特细胞定位表明它们在血细胞生成，组织重塑或作为内源性凝集素的免疫应答中的重要作用。

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