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首页> 外文期刊>Horticulture,Environment,and Biotechnology >Quantitative trait loci analysis of flowering time and vegetative traits in tomato plants grown using different seedling raising methods.
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Quantitative trait loci analysis of flowering time and vegetative traits in tomato plants grown using different seedling raising methods.

机译:采用不同育苗方法生长的番茄植物开花时间和营养性状的数量性状位点分析。

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Several quantitative trait loci (QTL) analysis have been conducted on the flowering time of tomato plants, but only few studies have examined both epistatic interaction between QTLs and QTL x environment interaction effects. Using a mixed linear-model composite-interval mapping technique, we have identified additive and the epistatic QTLs that control days to flowering (DTF), number of leaves before the first inflorescence (LN), plant height (PH), length of largest leaf (LL), number of lateral shoots (LS) and shoot fresh weight (SFW) in tomato plants cultivated using two seedling raising methods (raising in 128-cell trays with subsequent transplantation or cultivation in 1 L pots). Two experiments were conducted in autumn 2007 and spring 2009. Cell-raised plants flowered later than pot-raised plants in both experiments, and environment effects were significant for flowering time. However, the genotype x environment interaction at the phenotypic level was significant only in autumn 2007. Eight additive QTLs and nine epistatic QTL pairs were detected for DTF and LN, and eight additive QTLs and six epistatic interactions were detected for the vegetative traits. Only one additive QTL (ll1a) and an epistatic QTL pair (sfw1-sfw5) exhibited QTL x environment interactions. Most of the epistatic QTLs identified in this study did not have additive effects. Flowering-time QTLs that did not have QTL x environment interaction effects may be nonspecific QTLs, i.e., QTLs not affected by internal or external factors, which are desirable for marker-assisted breeding.
机译:已经对番茄植物的开花时间进行了一些数量性状基因座(QTL)分析,但是只有很少的研究研究了QTL之间的上位性相互作用和QTL x环境相互作用的影响。使用混合线性模型复合间隔映射技术,我们已经确定了添加剂和上位QTL,这些QTL控制开花天数(DTF),第一次开花前的叶子数(LN),植物高度(PH),最大叶的长度(LL),使用两种育苗方法栽培的番茄植株的侧枝数(LS)和枝条鲜重(SFW)(在128细胞托盘中进行育种,然后进行移植或在1 L盆中进行培养)。 2007年秋天和2009年春季进行了两个实验。在两个实验中,细胞培养的植物开花都比盆栽的开花晚,而且环境影响对于开花时间也很重要。然而,仅在2007年秋季,在表型水平上的基因型x环境相互作用才有意义。检测到8个添加QTL和9个上位QTL对的DTF和LN,以及8个添加QTL和6个上位相互作用的营养性状。只有一个加性QTL( ll1a )和上位QTL对( sfw1-sfw5 )表现出QTL x环境相互作用。在这项研究中确定的大多数上位QTL不具有累加作用。没有QTL x环境相互作用影响的开花期QTL可能是非特异性QTL,即不受内部或外部因素影响的QTL,这是标记辅助育种所希望的。

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