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Development of a Nutrient Film Technique Culture System for Arbuscular Mycorrhizal Plants

机译：丛枝菌根植物营养膜技术培养系统的研制

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A nutrient film technique (NFT) culture system was developed to allow nursery production of arbuscular mycorrhizal horticultural crops. This would benefit horticultural production and allow for uncomplicated production of mycorrhizal hyphae. Roots oflettuce (Lactuca sativa var. capitata) plants were highly colonized by the arbuscular mycorrhizal fungus, Glomus mosseae (BEG 107) after 4 weeks in the NFT system, following an initial phase of five weeks in inoculated in Perlite substrate. In the NFT system, a thin layer of glass beads was used to provide solid support for plant and fungus growth and nutrient solution was supplied intermittently (15 min, six times per day). A modified nutrient solution (80 ^M P) was used and was replaced with fresh solution every 3 days. A significantly higher dry weight was found for the mycorrhizal versus the nonmycorrhizal lettuce plants in Perlite during the precolonization period. The root colonization rate was also high at rates up to 80 U.M P supply. On the NFTsystem, growth differences between mycorrhizal and nonmycorrhizal plants were less than in Perlite. However, root colonization rate was not reduced during the NFT culture period. In this system, high amounts of fungal biomass were produced. This would allow the determination of metal and other nutrient concentrations in fungal hyphae. Furthermore, we found large amounts of external fungal hyphae surrounding the root surface. As much as 130 mg fungal biomass were collected per culture plate (three plants). Therefore, we suggest that this modified NFT culture system would be suitable for fungal biomass production on a large scale with a view to additional aeration by intermittent nutrient supply, optimum Psupply, and a use of glass beads as support materials. Furthermore, bulk inoculum composition with a mixture of spores, colonized roots, and hyphae grown in soilless media by the modified NFT system might be a useful way to massproduce mycorrhizal crops and inoculum for commercial horticultural purposes.
机译：开发了一种营养膜技术（NFT）培养系统，以允许苗圃生产丛枝菌根园艺作物。这将有利于园艺生产并允许菌根菌丝的简单生产。在NFT系统中接种4周后，在珍珠岩基质中接种了5周的初始阶段后，丛枝菌根真菌Glomus mosseae（BEG 107）将莴苣的根生莴苣（Lactuca sativa var。capitata）植物高度定殖。在NFT系统中，玻璃珠的薄层用于为植物和真菌的生长提供固体支持，并间歇性地（每天六次，每次15分钟）提供营养液。使用改良的营养液（80μMP），每隔3天用新鲜溶液替换一次。在定殖前，珍珠岩中的菌根生菜和非菌根生菜植株的干重明显增加。根定殖率也很高，最高供应量为80 U.MP。在NFT系统上，菌根和非菌根植物之间的生长差异小于珍珠岩。但是，在NFT培养期间，根部定植率并未降低。在该系统中，产生了大量的真菌生物质。这样可以确定真菌菌丝中金属和其他营养物的浓度。此外，我们发现根表面周围有大量外部真菌菌丝。每个培养板（三株植物）收集了多达130 mg的真菌生物质。因此，我们建议这种改良的NFT培养系统将适合大规模生产真菌生物质，以期通过间歇性营养供应，最佳Psupply供应和使用玻璃珠作为支撑材料来增加通气。此外，通过改良的NFT系统在无土培养基中生长的散装接种物组合物具有孢子，定殖根和菌丝的混合物，可能是批量生产用于商业园艺目的的菌根作物和接种物的有用方法。

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