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首页> 外文期刊>Human Immunology: Official Journal of the American Society for Histocompatibility and Immunogenetics >HLAMatchmaker: a molecularly based algorithm for histocompatibility determination. V. Eplet matching for HLA-DR, HLA-DQ, and HLA-DP.
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HLAMatchmaker: a molecularly based algorithm for histocompatibility determination. V. Eplet matching for HLA-DR, HLA-DQ, and HLA-DP.

机译：HLAMatchmaker：一种用于组织相容性确定的基于分子的算法。 V. HLA-DR，HLA-DQ和HLA-DP的Eplet匹配。

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摘要

This report describes the design of the eplet version of HLAMatchmaker to determine class II compatibility at the structural level. This matching algorithm is based on the hypothesis, developed from molecular modeling of crystallized antigen-antibody complexes, that functional epitopes are represented by patches of surface-exposed nonself-amino acid residues surrounded by residues within a 3-A radius. Patch determinations with a molecular viewer of crystalline structural models downloaded from the Entrez Molecular Modeling Database Web site led to the identification of 44 DRB, 33DQB, 29 DQA, 20 DPB, and 9 DPA unique combinations of polymorphic positions. The residue compositions of these patches were then determined from amino acid sequences. This analysis resulted in a repertoire of 146 DRB, 74 DQB, 58 DQA, 45 DPB, and 19 DPA eplets. In many eplets, the residues are in short linear sequences, but many other eplets have discontinuous sequences of residues that cluster on or near the molecular surface. This analysis has also shown that all serologically defined DR and DQ antigens detectable by monospecific antibodies have unique eplets. Other eplets are present in groups of class II antigens, many of which appear as cross-reacting. The eplet version of HLAMatchmaker should be considered as a hypothetical model for the structural assessment of donor-recipient compatibility and the determination of mismatch acceptability for sensitized patients. This computer algorithm can be downloaded from the HLA Matchmaker Webside at http://tpis.upmc.edu.
机译：该报告描述了HLAMatchmaker的eplet版本的设计，以确定在结构级别上的II类兼容性。该匹配算法基于从结晶的抗原-抗体复合物的分子模型发展而来的假设，即功能性表位由表面暴露的非自身氨基酸残基的斑块表示，这些残基被3-A半径内的残基包围。使用从Entrez分子模型数据库网站下载的晶体结构模型的分子查看器进行补丁确定，可以识别出44个DRB，33DQB，29 DQA，20 DPB和9 DPA多态性位置的独特组合。然后从氨基酸序列确定这些贴剂的残基组成。该分析得出了146个DRB，74个DQB，58个DQA，45个DPB和19个DPA eplet的库。在许多小片段中，残基是短的线性序列，但是许多其他小片段具有在分子表面上或附近聚集的残基的不连续序列。该分析还表明，可被单特异性抗体检测到的所有血清学定义的DR和DQ抗原均具有独特的小片段。 II类抗原组中还存在其他小片段，其中许多以交叉反应的形式出现。 HLAMatchmaker的eplet版本应被视为用于评估供体-受体相容性和确定致敏患者错配可接受性的假设模型。可以从HLA Matchmaker网站上的http://tpis.upmc.edu下载此计算机算法。

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