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首页> 外文期刊>Human Pathology >Expression and clinical role of DJ-1, a negative regulator of PTEN, in ovarian carcinoma.
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Expression and clinical role of DJ-1, a negative regulator of PTEN, in ovarian carcinoma.

机译：PTEN负调节剂DJ-1在卵巢癌中的表达及其临床作用。

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摘要

The aim of this study was to analyze the expression and clinical role of DJ-1, a negative regulator of PTEN (phosphatase and tensin homolog deleted on chromosome 10), in ovarian carcinoma, and investigate the putative association between DJ-1 levels and expression of its transcriptional regulators specificity protein 1 (Sp1) and specificity protein 3 (Sp3). Effusions (n = 72) and solid tumors (n = 57, 42 primary and 15 metastases) were analyzed for DJ-1 messenger RNA (mRNA) expression using reverse transcriptase-polymerase chain reaction. Most specimens (48 effusions, 50 solid tumors) were additionally analyzed for Sp1 and Sp3 mRNA expression. PTEN protein expression was analyzed in 201 effusions and 92 solid tumors using immunohistochemistry. DJ-1 mRNA was expressed in more than 80% of specimens, with no preferential anatomical site. DJ-1 expression was positively associated with Sp1 expression in effusions (P = .03) and with Sp1 (P = .02) and Sp3 (P = .002) expression in solid tumors. In effusions, DJ-1 expression was higher in postchemotherapy compared with prechemotherapy specimens (P = .012). Higher DJ-1 levels (P = .027) and more advanced FIGO stage (IV versus III; P = .003) correlated with shorter progression-free survival in univariate analysis for patients with postchemotherapy effusions. PTEN expression was low in effusions and solid tumors (23% and 13%, respectively), and its expression showed no association with DJ-1 levels or survival. Our data show that DJ-1 is frequently expressed in advanced-stage ovarian carcinoma at all anatomical sites and is coexpressed with its transcriptional regulators Sp1 and Sp3. In contrast, PTEN expression is infrequent in this disease. These findings may provide one of the molecular mechanisms that mediate cancer cell survival and aggressiveness in this tumor.
机译：这项研究的目的是分析卵巢癌中PTEN的负调控因子DJ-1（磷酸酶和张力蛋白同源物在10号染色体上缺失）的表达及其临床作用，并研究DJ-1水平与表达之间的推测联系转录调节蛋白特异性蛋白1（Sp1）和特异性蛋白3（Sp3）的表达。使用逆转录聚合酶链反应分析渗出液（n = 72）和实体瘤（n = 57、42个原发灶和15个转移灶）的DJ-1信使RNA（mRNA）表达。另外分析了大多数标本（48次积液，50个实体瘤）的Sp1和Sp3 mRNA表达。使用免疫组织化学分析了201例积液和92例实体瘤中的PTEN蛋白表达。 DJ-1 mRNA在超过80％的标本中表达，没有优先的解剖部位。 DJ-1表达与积液中的Sp1表达呈正相关（P = .03），实体瘤中的Sp1（P = .02）和Sp3（P = .002）表达呈正相关。积液中，DJ-1在化疗后的表达高于化疗前的标本（P = 0.012）。在化疗后积液患者的单因素分析中，较高的DJ-1水平（P = .027）和较高的FIGO阶段（IV对III; P = .003）与较短的无进展生存期相关。在积液和实体瘤中，PTEN表达较低（分别为23％和13％），并且其表达与DJ-1水平或存活率无关。我们的数据显示，DJ-1在所有解剖部位的晚期卵巢癌中频繁表达，并与其转录调节因子Sp1和Sp3共表达。相反，在该疾病中PTEN表达很少。这些发现可能提供介导该肿瘤中癌细胞存活和侵袭性的分子机制之一。

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