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首页> 外文期刊>Human Reproduction >Protamine 2 precursors, protamine 1/protamine 2 ratio, DNA integrity and other sperm parameters in infertile patients.
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Protamine 2 precursors, protamine 1/protamine 2 ratio, DNA integrity and other sperm parameters in infertile patients.

机译：不育患者中鱼精蛋白2的前体，鱼精蛋白1 /鱼精蛋白2的比例，DNA完整性和其他精子参数。

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BACKGROUND: The protamine 1-to-protamine 2 ratio (P1/P2) is altered in the sperm cells of some infertile patients. Also, evidence for increased protamine 2 precursors (pre-P2) in a few patients has been reported. But so far, there have been no studies measuring simultaneously these two variables in a large number of patients. METHODS: We measured the P1/P2 ratio and the presence of pre-P2 using, for the first time, an antibody specific to the precursor pre-P2, together with other sperm parameters in 224 infertile patients. Additionally, the DNA integrity was assessed by terminal transferase dUTP nick-end labelling (TUNEL) in a subset of the samples. RESULTS: Pre-P2 levels show a significant positive correlation with the P1/P2 ratio, with the presence of other proteins and, at low pre-P2 levels, with TUNEL-positive sperm. An inverse correlation with sperm count, normal morphology and motility was detected. CONCLUSIONS: The levels of pre-P2 may provide clues into the pathogenic mechanisms of infertility.The increased proportion of pre-P2 in some patients with increased P1/P2 ratio suggests an involvement of pre-P2 processing. The positive correlation between TUNEL-positive sperm and pre-P2 at low pre-P2/P2 ratios also suggests a link between deficient protamine processing and decreased DNA integrity.
机译：背景：在一些不育患者的精子细胞中，鱼精蛋白1与鱼精蛋白2的比率（P1 / P2）发生了变化。另外，已经报道了一些患者中鱼精蛋白2前体（pre-P2）增加的证据。但是到目前为止，还没有研究在大量患者中同时测量这两个变量。方法：我们首次使用前体前P2特异抗体和224例不育患者的其他精子参数，测量了P1 / P2比率和前P2的存在。此外，通过部分样品中的末端转移酶dUTP缺口末端标记（TUNEL）评估了DNA的完整性。结果：P2之前的水平与P1 / P2比率，其他蛋白质的存在以及TUNEL阳性精子的存在呈显着正相关。与精子数量，正常形态和运动力呈负相关。结论：P2前体的水平可能为不孕症的发病机制提供了线索。P1/ P2比增加的某些患者中P2前体的比例增加表明P2前体的参与。 TUNEL阳性精子和pre-P2之间的正相关性在低的P2 / P2比率下也表明鱼精蛋白加工不足与DNA完整性降低之间存在联系。

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