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首页> 外文期刊>Human Reproduction >Implantation-associated gene-1 (Iag-1): a novel gene involved in the early process of embryonic implantation in rat.
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Implantation-associated gene-1 (Iag-1): a novel gene involved in the early process of embryonic implantation in rat.

机译：植入相关基因1（Iag-1）：一种参与大鼠胚胎植入早期过程的新基因。

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摘要

BACKGROUND: In order to study the novel genes related to rat embryonic implantation, a novel implantation-associated gene, Iag-1, was identified and characterized from rat uterus of early pregnancy. Iag-1 was initially derived from suppressive subtracted hybridization of a cDNA library of rat uterus, which was used to analyse differentially expressed genes between the preimplantation and implantation period. METHODS: The full-length cDNA sequence of Iag-1 was cloned from rat uterus on D5.5 of pregnancy by 5'- and 3'-RACE. The expression of Iag-1 in the uterus of early pregnancy, pseudopregnancy, artificial decidualization and activation of delayed implantation was detected by northern blotting, in situ hybridization, western blotting and immunofluorescence. Endometrial stromal cells (ESCs) were isolated from rat uterus. The effect of Iag-1 on ESCs proliferation and apoptosis were determined by MTT assay, TUNEL and Hoechst staining. Apoptosis-related proteins in ESCs were detected by western blotting. RESULTS: Differential patterns of Iag-1 expression were detected in rat embryo and in the uterus during the peri-implantation period. Iag-1 was specifically localized in glandular epithelium and luminal epithelium. In contrast, the expression of Iag-1 was not significantly altered in uterus of pseudopregnancy and artificial decidualization, but was significantly increased in the uterus after activation of delayed implantation. Stable expression of introduced Iag-1 inhibited the proliferation of in vitro-cultured ESCs. Significant apoptosis was also detected in the ESCs overexpressing Iag-1, along with the enhancement of p53 and Bax protein expression. CONCLUSIONS: Overexpression of Iag-1 can inhibit ESCs proliferation and induce ESCs apoptosis, and p53 and Bax may play an important role in the process of Iag-1-induced apoptosis.
机译：背景：为了研究与大鼠胚胎着床有关的新基因，从早期妊娠的大鼠子宫中鉴定出了一种与胚胎着床有关的新基因Iag-1。 Iag-1最初源自大鼠子宫cDNA文库的抑制性消减杂交，该杂交用于分析植入前和植入期之间差异表达的基因。方法：在妊娠D5.5通过5'-和3'-RACE从大鼠子宫中克隆出Iag-1的全长cDNA序列。通过Northern印迹，原位杂交，Western印迹和免疫荧光检测Iag-1在早孕，假孕，人工蜕膜和延迟植入的子宫中的表达。从大鼠子宫分离出子宫内膜基质细胞（ESCs）。用MTT法，TUNEL法和Hoechst染色法测定Iag-1对ESCs增殖和凋亡的影响。通过蛋白质印迹法检测ESC中与凋亡相关的蛋白。结果：在植入前后的大鼠胚胎和子宫中均检测到Iag-1表达的差异。 Iag-1专门定位在腺上皮和管腔上皮中。相反，假妊娠和人工蜕膜化子宫中Iag-1的表达没有明显改变，但延迟植入激活后子宫中Iag-1的表达明显增加。导入的Iag-1的稳定表达抑制了体外培养的ESC的增殖。在过表达Iag-1的ESC中也检测到显着的细胞凋亡，以及p53和Bax蛋白表达的增强。结论：Iag-1的过表达可抑制ESC的增殖并诱导ESC的凋亡，p53和Bax可能在Iag-1诱导的凋亡过程中起重要作用。

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