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首页> 外文期刊>Human Reproduction >Epidermal growth factor effects on marmoset monkey (Callithrix jacchus) oocyte in vitro maturation, IVF and embryo development are altered by gonadotrophin concentration during oocyte maturation.
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Epidermal growth factor effects on marmoset monkey (Callithrix jacchus) oocyte in vitro maturation, IVF and embryo development are altered by gonadotrophin concentration during oocyte maturation.

机译：表皮生长因子对mar猴卵体外成熟的影响，卵母细胞成熟过程中的促性腺激素浓度改变了IVF和胚胎发育。

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BACKGROUND: This is the first study of the effect of epidermal growth factor (EGF) on marmoset monkey oocytes matured in vitro. METHODS: We have evaluated the effects of 10 ng/ml EGF in combination with 1 or 10 IU/ml of gonadotrophins (FSH/hCG 1:1 ratio) during in vitro maturation (IVM) of marmoset oocytes. Immature cumulus-oocyte complexes (COCs) were retrieved from ovarian antral follicles of unprimed monkeys. COCs from six animals (n= 268) used in this study were randomly distributed among four experimental groups: (A) 1 FSH +1 hCG; (B) 10 FSH +10 hCG; (C) 1 FSH +1 hCG + EGF; and (D) 10 FSH +10 hCG + EGF (where 1 and 10 are concentrations, IU/ml). After IVM, oocytes were fertilized in vitro and embryos were allowed to progress up to 87-88 h. RESULTS: the highest rate of total and radial cumulus expansion was observed in Group A, with the lowest in Group B (P < 0.05). Neither maturation nor fertilization rate were affected by gonadotrophin concentration or presence of EGF. Addition of EGF increased degeneration and decreased first cleavage rate, which was significantly lower in Group C than Group A (P < 0.005). Interestingly, in the EGF groups some embryos cleaved faster than without EGF. CONCLUSIONS: The effects of EGF are highly dependent on concentration of gonadotrophins present in IVM medium. EGF has a negative effect on oocytes in the presence of low gonadotrophins, but contrastingly partially protects oocytes from the negative effects of high gonadotrophins. We propose that these observed negative effects of EGF may suggest use of an inappropriate dose of growth factor.
机译：背景：这是表皮生长因子（EGF）对在体外成熟的mar猴卵母细胞的作用的首次研究。方法：我们评估了mar猴卵母细胞体外成熟（IVM）过程中10 ng / ml EGF与1或10 IU / ml促性腺激素（FSH / hCG 1：1比）的作用。从未灌注猴子的卵巢窦卵泡中回收未成熟的卵-卵母细胞复合物（COC）。本研究中使用的六只动物（n = 268）的COC随机分布在四个实验组中：（A）1 FSH +1 hCG; （B）10 FSH +10 hCG; （C）1个FSH +1 hCG + EGF; （D）10 FSH +10 hCG + EGF（其中1和10是浓度，IU / ml）。 IVM后，卵母细胞体外受精，使胚胎发育长达87-88小时。结果：A组的总和radial积扩张率最高，B组最低（P <0.05）。促性腺激素浓度或EGF的存在都不会影响成熟度和受精率。添加EGF会增加变性并降低首次卵裂率，C组明显低于A组（P <0.005）。有趣的是，在EGF组中，某些胚胎的分裂速度要快于没有EGF的情况。结论：EGF的作用高度依赖于IVM培养基中存在的促性腺激素浓度。在存在低促性腺激素的情况下，EGF对卵母细胞具有负面影响，但相反，部分保护了卵母细胞免受高促性腺激素的负面影响。我们建议这些观察到的EGF负面影响可能表明使用了不合适剂量的生长因子。

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