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首页> 外文期刊>Human and Experimental Toxicology >Antiproliferation and anti-migration induced by gypenosides in human colon cancer SW620 and esophageal cancer Eca-109 cells.
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Antiproliferation and anti-migration induced by gypenosides in human colon cancer SW620 and esophageal cancer Eca-109 cells.

机译：绞股蓝皂苷在人结肠癌SW620和食道癌Eca-109细胞中诱导的抗增殖和抗迁移。

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摘要

Gypenosides (Gyp), found in Gynostemma pentaphyllum Makino, has attracted more attention owing to its wide bioactivities. However, the effects of Gyp on esophageal cancer cells and colon cancer cells are still unknown. The present study was to investigate the possible anti-proliferative and anti-migration activity of Gyp on human colon cancer cells SW620 and esophageal cancer cells Eca-109. Cell viability was evaluated using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cell membrane integrity was evaluated using flow cytometry following propidium iodide staining. Apoptotic cell death was determined by nuclear 4'-6-diamidino-2-phenylindole staining. Generation of intracellular reactive oxygen species (ROS) and changes in mitochondrial membrane potential (Δψ m) was analyzed by flow cytometry using 2',7'-dichlorofluorescein-diacetate and rhodamine 123 staining, respectively. Wound healing assay was carried out to investigate Gyp-inhibited migration of SW620 and Eca-109 cells. The results indicated that Gyp inhibited cell proliferation and migration in SW620 and Eca-109 cells in dose- and time-dependent manner. Gyp elevated intracellular ROS level, decreased the Δψ m, and induced apoptotic morphology such as cell shrinkage and chromatin condensation, suggesting oxidative stress and mitochondria-dependent cell apoptosis that might be involved in Gyp-induced cell viability loss in SW620 and Eca-109 cells. The findings indicate Gyp may have valuable application in clinical colon cancer and esophageal cancer treatments.
机译：绞股蓝（Gynostemma pentaphyllum Makino）中发现的绞股蓝总皂甙（Gyp），由于其广泛的生物活性而备受关注。然而，Gyp对食道癌细胞和结肠癌细胞的作用仍是未知的。本研究旨在研究Gyp对人结肠癌细胞SW620和食道癌细胞Eca-109的可能的抗增殖和抗迁移活性。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑（MTT）分析评估细胞活力。碘化丙锭染色后，使用流式细胞仪评估细胞膜的完整性。通过核4'-6-二mid基-2-苯基吲哚染色确定凋亡细胞死亡。通过流式细胞术分别使用2'，7'-二氯荧光素-二乙酸盐和若丹明123染色，通过流式细胞术分析了细胞内活性氧（ROS）的产生和线粒体膜电位的变化（Δψm）。进行伤口愈合测定以研究Gyp抑制的SW620和Eca-109细胞的迁移。结果表明，Gyp以剂量和时间依赖性方式抑制SW620和Eca-109细胞的增殖和迁移。 Gyp升高细胞内ROS水平，降低Δψm，并诱导凋亡形态，例如细胞收缩和染色质浓缩，提示氧化应激和线粒体依赖性细胞凋亡可能与Gyp诱导SW620和Eca-109细胞的细胞活力丧失有关。这些发现表明，Gyp在临床结肠癌和食道癌治疗中可能具有有价值的应用。

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