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首页> 外文期刊>Human and Experimental Toxicology >Application of ultrasound-assisted emulsification microextraction for simultaneous determination of aminophenol isomers in human urine, hair dye, and water samples using high-performance liquid chromatography
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Application of ultrasound-assisted emulsification microextraction for simultaneous determination of aminophenol isomers in human urine, hair dye, and water samples using high-performance liquid chromatography

机译：超声辅助乳化微萃取用于高效液相色谱法同时测定人尿液，染发剂和水样中的氨基酚异构体

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摘要

Aminophenol isomers (2-, 3-, and 4-aminophenols) are typically classified as industrial pollutants with genotoxic and mutagenic effects due to their easy penetration through the skin and membranes of human, animals, and plants. In the present study, a simple and efficient ultrasound-assisted emulsification microextraction procedure coupled with high-performance liquid chromatography with ultraviolet detector was developed for preconcentration and determination of these compounds in human fluid and environmental water samples. Effective parameters (such as type and volume of extraction solvent, pH and ionic strength of sample, and ultrasonication and centrifuging time) were investigated and optimized. Under optimum conditions (including sample volume: 5 mL; extraction solvent: chloroform, 80 μL; pH: 6.5; without salt addition; ultrasonication: 3.5 min; and centrifuging time: 3 min, 5000 rpm min-1), the enrichment factors and limits of detection were ranged from 42 to 51 and 0.028 to 0.112 μg mL-1, respectively. Once optimized, analytical performance of the method was studied in terms of linearity (0.085-157 μg mL-1, r2 > 0.998), accuracy (recovery = 88.6- 101.7%), and precision (repeatability: intraday precision < 3.98%, and interday precision < 5.12%). Finally, applicability of the method was evaluated by the extraction and determination of these compounds in human urine, hair dye, and real water samples.
机译：氨基苯酚异构体（2-，3-和4-氨基苯酚）由于易于穿透人，动物和植物的皮肤和膜，通常被归类为具有遗传毒性和诱变作用的工业污染物。在本研究中，开发了一种简单高效的超声辅助乳化微萃取程序，并结合带有紫外检测器的高效液相色谱仪，用于对人体液体和环境水样品中的这些化合物进行预浓缩和测定。研究并优化了有效参数（例如萃取溶剂的类型和体积，样品的pH值和离子强度以及超声处理和离心时间）。在最佳条件下（包括样品量：5 mL;萃取溶剂：氯仿，80μL; pH：6.5;不加盐;超声：3.5 min;离心时间：3 min，5000 rpm min-1），富集因子和检测限分别为42至51和0.028至0.112μgmL-1。优化后，就线性（0.085-157μgmL-1，r2> 0.998），准确性（回收率= 88.6- 101.7％）和准确性（可重复性：日内精度<3.98％，和日内精确度<5.12％）。最后，通过提取和测定人尿，染发剂和真水样品中的这些化合物来评估该方法的适用性。

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