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首页> 外文期刊>Zoonoses and Public Health >Prevalence, Antimicrobial Resistance and Risk Factors for Thermophilic Campylobacter Infections in Symptomatic and Asymptomatic Humans in Tanzania
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Prevalence, Antimicrobial Resistance and Risk Factors for Thermophilic Campylobacter Infections in Symptomatic and Asymptomatic Humans in Tanzania

机译：坦桑尼亚有症状和无症状人类嗜热弯曲杆菌感染的患病率，抗菌素耐药性和危险因素

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The genus Campylobacter comprises members known to be a leading cause of foodborne gastrointestinal illness worldwide. A study was conducted to determine the epidemiology and antimicrobial resistance of Campylobacter in humans in Morogoro, Eastern Tanzania. Isolation of Campylobacter from stool specimens adopted the Cape Town protocol. Campylobacter isolates were preliminarily identified by conventional phenotypic tests and subsequently confirmed by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry and polymerase chain reaction. Antimicrobial resistance testing employed the disc diffusion method. A small proportion of the test isolates was also subjected to agar dilution method. Risk factors for human illness were determined in an unmatched case-control study. Thermophilic Campylobacter were isolated from 11.4% of the screened individuals (n=1195). The agreement between PCR and MALDI-TOF was perfect (=1.0). Symptomatics and young individuals were infected with higher numbers than asymptomatic and adults, respectively. The majority (84.6%) of the isolates were C.jejuni and the remaining were C.coli. Isolates had highest resistance (95.6%) for colistin sulphate and lowest for ciprofloxacin (22.1%). The rates of resistance for other antibiotics (azithromycin, erythromycin, tetracycline, cephalothin, gentamycin, nalidixic acid, ampicillin, amoxycillin, norfloxacin, chloramphenicol) ranged from 44.1% to 89%. Comparison between disc diffusion and agar dilution methods indicated a good correlation, and the tests were in agreement to each other (0.75). Human illness was found to be associated with young age and consumption of chicken meat and pre-prepared salad. Our data indicate the presence of antibiotic-resistant thermophilic Campylobacter in humans in the study area. There is a need for routine investigation of the presence of theorganisms in gastroenteritis aetiology, including determination of their antibiotic susceptibilities.
机译：弯曲杆菌属包括已知为全世界食源性胃肠疾病的主要原因的成员。进行了一项研究，以确定坦桑尼亚东部莫罗哥罗的弯曲杆菌的流行病学和抗菌素耐药性。从粪便标本中分离弯曲杆菌，采用开普敦方案。弯曲杆菌分离株通过常规的表型测试进行了初步鉴定，随后通过基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱和聚合酶链反应进行了确认。抗菌剂耐药性测试采用盘片扩散法。少量测试分离株也进行了琼脂稀释法。在一项无与伦比的病例对照研究中确定了人类疾病的危险因素。从11.4％的筛查个体中分离出嗜热弯曲杆菌（n = 1195）。 PCR和MALDI-TOF之间的一致性非常好（= 1.0）。有症状者和年轻人感染率分别高于无症状者和成年人。分离株的大部分（84.6％）为空肠弯曲杆菌，其余为大肠杆菌。分离株对硫酸粘菌素的耐药性最高（95.6％），对环丙沙星的耐药性最低（22.1％）。对其他抗生素（阿奇霉素，红霉素，四环素，头孢菌素，庆大霉素，萘啶酸，氨苄青霉素，阿莫西林，诺氟沙星，氯霉素）的耐药率在44.1％至89％之间。椎间盘扩散法和琼脂稀释法之间的比较显示出良好的相关性，并且测试彼此一致（0.75）。人们发现人类疾病与年轻，食用鸡肉和预先准备的沙拉有关。我们的数据表明在研究区域的人类中存在耐抗生素的嗜热弯曲杆菌。有必要对胃肠炎病因中微生物的存在进行常规调查，包括确定其抗生素敏感性。

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