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首页> 外文期刊>Zoological Science >Cloning and expression of a cDNA encoding a prolactin receptor of theJapanese red-bellied newt, Cynops pyrrhogaster
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Cloning and expression of a cDNA encoding a prolactin receptor of theJapanese red-bellied newt, Cynops pyrrhogaster

机译：日本红腹newCynops pyrrhogaster催乳素受体的cDNA的克隆和表达

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摘要

Primary structure of a newt prolactin receptor (nPRL-R) was deduced from a cDNA clone isolated from a kidney cDNA library as well as from a polymerase chain reaction (PCR) product. The predicted nPRL-R protein was composed of 626 amino acids (aa), and contained a signal sequence and a transmembrane region. The extracellular domain had two pairs of cysteine residues and a WSXWS motif. The cytoplasmic domain comprised 368 residues and contained both box 1 and box 2 sequences which are considered to be required for the signal transduction of the cytokine/growth hormone (GH)/PRL-R family in mammals. The nPRL-R shares 50-52% protein sequence identity with mammalian PRL-Rs. When nPRL-R was expressed in COS-7 cells, specific binding of [I-125] rat prolactin (PRL) was observed. Northern blot analysis revealed the existence of a single transcript, more than 10 kb in length, which was expressed in the kidney and brain. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed the expression of PRL-R mRNA in the testis, ovary, liver and bladder of the newt. This is, as far as we know, the first report of cloning on amphibian PRL-R.
机译：t催乳素受体（nPRL-R）的一级结构是从肾脏cDNA文库和聚合酶链反应（PCR）产物中分离出的cDNA克隆推导出来的。预测的nPRL-R蛋白由626个氨基酸（aa）组成，并包含信号序列和跨膜区。细胞外结构域具有两对半胱氨酸残基和一个WSXWS基序。细胞质结构域包含368个残基，并包含框1和框2序列，这被认为是哺乳动物细胞因子/生长激素（GH）/ PRL-R家族信号转导所必需的。 nPRL-R与哺乳动物PRL-R共享50-52％的蛋白质序列同一性。当nPRL-R在COS-7细胞中表达时，观察到[I-125]大鼠催乳素（PRL）的特异性结合。 Northern印迹分析揭示了存在于肾脏和大脑中的单个转录本，其长度超过10 kb。逆转录-聚合酶链反应（RT-PCR）分析显示PRL-R mRNA在the的睾丸，卵巢，肝和膀胱中表达。据我们所知，这是两栖动物PRL-R克隆的第一个报告。

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