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首页> 外文期刊>Zoological Science >The cDNA sequence and expression of the AAA-family peroxin genes pex-1 andpex-6 from the nematode Caenorhabditis elegans
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The cDNA sequence and expression of the AAA-family peroxin genes pex-1 andpex-6 from the nematode Caenorhabditis elegans

机译：线虫秀丽隐杆线虫AAA家族过氧化物酶基因pex-1和pex-6的cDNA序列和表达

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摘要

We cloned cDNAs encoding two peroxins, PEX-1 and PEX-6, of the nematode Caenorhabditis elegans. Peroxins are proteins that play essential roles in peroxisome biogenesis and are encoded by pex genes. Among the peroxins, PEX-1 and PEX-6 constitute the subfamily 2 of AAA (ATPases associated with diverse cellular activities) proteins. Each cDNA agreed well with the respective mRNA in size (3.4 kb for pex-1 and 2.3 kb for pex-6) and did not carry any spliced leader sequence. The pex-1 cDNA was composed of 24 exons, which were encoded by a genomic region containing three open reading frames (ORFs), c11h1.4, c11h1.5, and c11h1.6; the predicted ORF c11h1.5 was encompassed in the 15th intron. Although many exon-intron borders in pex-1 were inconsistent with those predicted for c11h1.4 and c11h1.6, those in pex-6 coincided with those for the ORF f39g3.7. The pex-1 and pex-6 genes encoded proteins with 996 and 720 amino acid residues, respectively. Both pex-1 mRNA and pex-6 mRNA were detectable mainly in intestinal cells throughout the life cycle of C. elegans.
机译：我们克隆了线虫秀丽隐杆线虫的两个过氧化物酶PEX-1和PEX-6的cDNA编码。过氧化物是在过氧化物酶体生物发生中起重要作用的蛋白质，并由pex基因编码。在过氧化物酶中，PEX-1和PEX-6构成AAA（与多种细胞活性有关的ATPase）蛋白的亚家族2。每个cDNA与各自的mRNA大小一致（pex-1为3.4 kb，pex-6为2.3 kb），并且不带有任何剪接的前导序列。 pex-1 cDNA由24个外显子组成，它们由一个包含三个开放阅读框（ORF），c11h1.4，c11h1.5和c11h1.6的基因组区域编码。预测的ORF c11h1.5包含在第15个内含子中。尽管pex-1中的许多外显子-内含子边界与c11h1.4和c11h1.6的预测边界不一致，但pex-6中的边界与ORF f39g3.7的边界一致。 pex-1和pex-6基因分别编码具有996和720个氨基酸残基的蛋白质。在整个秀丽隐杆线虫的整个生命周期中，主要在肠细胞中都可以检测到pex-1 mRNA和pex-6 mRNA。

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