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首页> 外文期刊>Zoological Science >Cloning of molluscan telomere DNA with (TTAGGG)n repeat and itschromosomal location in the freshwater snail Biwamelania habei
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Cloning of molluscan telomere DNA with (TTAGGG)n repeat and itschromosomal location in the freshwater snail Biwamelania habei

机译：（TTAGGG）n重复序列的软体动物端粒DNA的克隆及其在淡水蜗牛Bewamelania habei中的染色体定位

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摘要

In the present study, we have cloned and determined the nucleotide sequence of telomeric DNA of a freshwater gastropod Biwamelania habei. The sequence was comprised of (TTAGGG)n tandem repeat, which is identical with the sequence of vertebrate telomere. The characteristic sequences, together with the chromosomal localization revealed by FISH analysis and the Bal 31 exonuclease sensitivity of the hybridization signal, support the sequences to be located at chromosomal ends. This is the first report for identification of molluscan telomere DNA and is also the first report of invertebrate telomere with (TTAGGG)n sequence. Although the telomere sequence of B. habei is different from that of any other invertebrates reported to date, the sequence universally occurs among various eukaryotes including some metazoans. It is thus evident that a. habei retains ancestral sequence for invertebrate telomeres. Interstitial telomeric signals were observed in some chromosomes. Some of such interstitial telomeric sites coincide with the presumed fusion point of Robertsonian rearrangement.
机译：在本研究中，我们已经克隆并确定了淡水腹足动物Biwamelania habei的端粒DNA的核苷酸序列。该序列由（TTAGGG）n串联重复组成，其与脊椎动物端粒的序列相同。特征序列，以及通过FISH分析揭示的染色体定位和杂交信号的Bal 31核酸外切酶敏感性，都支持将序列定位在染色体末端。这是鉴定软体动物端粒DNA的第一份报告，也是具有（TTAGGG）n序列的无脊椎动物端粒的第一份报告。尽管河北双歧杆菌的端粒序列不同于迄今报道的任何其他无脊椎动物的端粒序列，但该序列普遍存在于包括某些后生动物在内的各种真核生物中。因此，很明显habei保留了无脊椎动物端粒的祖先序列。在某些染色体中观察到间质性端粒信号。其中一些间质端粒位点与罗伯逊重排的推测融合点重合。

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