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首页> 外文期刊>Zoological Science >CNP is the only natriuretic peptide in an elasmobranch fish, Triakisscyllia
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CNP is the only natriuretic peptide in an elasmobranch fish, Triakisscyllia

机译：CNP是弹bra鱼类Triakisscyllia中唯一的利钠肽

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摘要

The natriuretic peptide (NP) family consists of endocrine ANP/BNP/VNP in the heart and paracrine CNP in the brain in vertebrates ranging from teleosts to mammals. In elasmobranchs, however, only CNP has been identified thus far in the heart and brain. To delineate the molecular evolution of this hormone family, it is essential to determine whether CNP is the only NP in this primitive fish group. In the present study, PCR cloning of all types of piscine NP was performed from the heart and brain of a dogfish, Triakis scyllia, using degenerate primers that amplified eel ANP, VNP and CNP. However, only CNP cDNA with an identical sequence was cloned from the heart and brain. Southern blot analysis showed that the CNP gene is a single copy gene, showing that endocrine CNP from the heart and paracrine CNP in the brain originate from the same gene. Since expression of the CNP gene was so high as demonstrated by Northern blot analysis, the abundantly expressed CNP mRNA could have interfered the amplification of other NP mRNAs expressed in small amounts. Therefore, a method was developed to cleave the cloned CNP mRNA specifically at the 3'-untranslated region with RNase H. After removal of the cloned CNP mRNA by this technique, no other NP cDNAs could be cloned, but small amounts of CNP cDNAs with shorter 3' sequence were amplified. These results strongly suggest that only CNP is present in elasmobranchs. Thus, it is likely that CNP is an ancestral form of the NP family and endocrine ANP/BNP/VNP have appeared later in the vertebrate evolution.
机译：利钠肽（NP）家族由从硬骨到哺乳动物的脊椎动物心脏中的内分泌ANP / BNP / VNP和大脑中的旁分泌CNP组成。然而，到目前为止，在弹性支中，在心脏和大脑中仅发现了CNP。为了描述该激素家族的分子进化，必须确定CNP是否是该原始鱼类中唯一的NP。在本研究中，使用简并引物扩增鳗鱼ANP，VNP和CNP，从狗鱼Triakis scyllia的心脏和大脑中进行了所有类型的鱼NP的PCR克隆。但是，仅从心脏和大脑克隆了具有相同序列的CNP cDNA。 Southern印迹分析表明CNP基因是单拷贝基因，表明来自心脏的内分泌CNP和大脑中的旁分泌CNP源自同一基因。由于通过Northern印迹分析证明CNP基因的表达如此之高，所以大量表达的CNP mRNA可能干扰了少量表达的其他NP mRNA的扩增。因此，开发了一种用RNase H在3'-非翻译区特异性切割克隆的CNP mRNA的方法。通过该技术去除克隆的CNP mRNA后，无法克隆其他NP cDNA，但少量的CNP cDNA具有较短的3'序列被扩增。这些结果强烈表明，弹性支中仅存在CNP。因此，CNP可能是NP家族的祖先形式，内分泌ANP / BNP / VNP出现在脊椎动物进化的后期。

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