...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Zoological Science >Vacuolar-type H+-ATPase and Na-, K--ATPase expression in gills of Atlantic salmon (Salmo salar) during isolated and combined exposure to hyperoxia and hypercapnia in fresh water.
【24h】

Vacuolar-type H+-ATPase and Na-, K--ATPase expression in gills of Atlantic salmon (Salmo salar) during isolated and combined exposure to hyperoxia and hypercapnia in fresh water.

机译：在淡水中高氧血症和高碳酸血症的单独和联合暴露期间，大西洋鲑（Salmo salar）g中的液泡型H + -ATPase和Na-，K-ATPase表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Changes in branchial vacuolar-type H+-ATPase B-subunit mRNA and Na+,K+-ATPase alpha- and beta-subunit mRNA and ATP hydrolytic activity were examined in smolting Atlantic salmon exposed to hyperoxic and/or hypercapnic fresh water. Pre-smolts, smolts, and post-smolts were exposed for 1 to 4 days to hyperoxia (100% O-2) and/or hypercapnia (2% CO2). Exposure to hypercapnic water for 4 days consistently decreased gill vacuolar-type H(+)ATPase B-subunit mRNA levels. Salmon exposed to hyperoxia had either decreased or unchanged levels of gill B-subunit mRNA. Combined hyperoxia+hypercapnia decreased B-subunit mRNA levels, although not to the same degree as hypercapnic treatment alone. Hyperoxia generally increased Na+,K+-ATPase alpha- and beta-subunit mRNA levels, whereas hypercapnia reduced mRNA levels in presmolts (beta) and smolts (alpha and beta). Despite these changes in mRNA levels, whole tissue Na+,K+-ATPase activity was generally unaffected by the experimental treatments. We suggest that the reduced expression of branchial vacuolar-type H+-ATPase B-subunit mRNA observed during internal hypercapnic acidosis may lead to reduction of functional V-type H+-ATPase abundance as a compensatory response in order to minimise intracellular HCO3- formation in epithelial cells.
机译：在熏制暴露于高氧和/或高碳酸血症淡水的大西洋鲑中，检查了分支液泡型H + -ATPase B亚基mRNA和Na +，K + -ATPaseα-和β-亚基mRNA的变化以及ATP水解活性。熏制前，熏制和熏制后暴露于高氧血症（100％O-2）和/或高碳酸血症（2％CO2）1-4天。暴露于高碳酸水4天一致降低consistently空泡型H（+）ATPase B亚基mRNA水平。暴露于高氧血症的鲑鱼的g B亚基mRNA含量降低或不变。高氧+高碳酸血症合并使用可降低B亚基mRNA水平，尽管程度与单独使用高碳酸血症治疗不同。高氧血症通常会增加Na +，K + -ATPaseα-和β-亚基mRNA的水平，而高碳酸血症会降低前性（β）和中性（α和β）的mRNA水平。尽管mRNA水平发生了这些变化，但整个组织的Na +，K + -ATPase活性通常不受实验处理的影响。我们建议内部高碳酸血症性酸中毒期间观察到的分支液泡型H + -ATPase B亚基mRNA的表达减少可能导致功能性V型H + -ATPase丰度的降低，这是一种代偿性反应，以使上皮细胞内HCO3-的形成最小化细胞。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号