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首页> 外文期刊>Zoological Science >Possible involvement of nitric oxide in signaling pigment dispersion in teleostean melanophores.
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Possible involvement of nitric oxide in signaling pigment dispersion in teleostean melanophores.

机译:一氧化氮可能参与硬骨鱼黑素细胞色素分散的信号。

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The possible involvement of nitric oxide (NO) in regulating the motile activities of teleostean melanophores was studied in the dark chub Zacco temmincki (Cyprinidae, Cypriniformes) and in the translucent glass catfish Kryptopterus bicirrhis (Siluridae, Siluriformes). NO donors, including (+/-)-(E)-methyl-2-[(E)hydroxyimino]-5-nitro-6-methoxy-3-hexaneamide (NOR 1), molsidomine (MSD), sodium nitroprusside (SNP) and glyceryl trinitrate (GTN), had no pigment-aggregating action on melanophores, but actively dispersed melanosomes in those cells. Among those reagents, NOR 1, a spontaneous releaser of NO, was the most effective. Inhibitors for nitric oxide synthase (NOS), i.e. Nomega-nitro-L-arginine methyl ester (L-NNA), Nomega-nitro-Larginine (L-NAME) and No)monomethyl-L-arginine (L-NMMA), showed melanosome-aggregating effects. A membrane-permeable analogue of cyclic guanosine-3',5'-monophosphate (8-Br-cGMP) was effective in dispersing melanosomes. The sum of these results suggests that NO plays an active role in the elaborate control of color changes in teleosts by dispersing pigment in melanophores via activation of soluble guanylyl cyclase to increase cytosolic levels of cGMP.
机译:在暗chuZacco temmincki(鲤科,鲤形目)和半透明的玻璃cat鱼rypto鱼(Cryptopterus bicirrhis)(Siluridae,Siluriformes)中,研究了一氧化氮(NO)可能参与调节硬骨鱼类的活动性。 NO供体,包括(+/-)-(E)-甲基-2-[((E)羟基亚氨基)-5-硝基-6-甲氧基-3-己酰胺(NOR 1),莫斯多明(MSD),硝普钠(SNP) )和三硝酸甘油酯(GTN)对黑素细胞没有色素聚集作用,但在这些细胞中活跃地分散了黑素体。在这些试剂中,NOR 1(NO的自发释放剂)是最有效的。一氧化氮合酶(NOS)抑制剂,即Nomega-硝基-L-精氨酸甲酯(L-NNA),Nomega-硝基-精氨酸(L-NAME)和No)一甲基-L-精氨酸(L-NMMA)黑素体聚集效应。环状鸟苷3',5'-单磷酸酯(8-Br-cGMP)的膜渗透性类似物可有效分散黑素体。这些结果的总和表明,NO通过激活可溶性鸟苷酸环化酶的作用将色素分散在黑色素团中,从而增加硬质骨的颜色变化,从而增加cGMP的胞浆水平,从而在精细控制硬骨鱼的颜色变化中起着积极作用。

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