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首页> 外文期刊>Zoological Science >Epidermal growth factor and transforming growth factor-alpha stimulate the proliferation of mouse uterine stromal cells
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Epidermal growth factor and transforming growth factor-alpha stimulate the proliferation of mouse uterine stromal cells

机译：表皮生长因子和转化生长因子-α刺激小鼠子宫基质细胞的增殖

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Growth factors produced in the uterine endometrium are considered to be involved in the proliferation of the mouse uterine stromal cells induced by estradiol-17beta (E-2) and progesterone (P). The effect of epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha), one of EGF-related growth factors, on the proliferation of mouse uterine stromal cells was studied in a serum-free culture. The growth of the uterine stromal cells was measured by MTT assay. EGF was found to increase the number of uterine stromal cells in a dose-dependent manner. The DNA-replicating cells were investigated using the immunocytochemical detection of bromodeoxyuridine (BrdU)-labeled cells. EGF and TGF-alpha increased the percentage of BrdU-Iabeled cells in a dose-dependent manner. Administration of the combination of E-2 (10(-9) M) and P (10(-7) M) for 2 days increased the percentage of BrdU-Iabeled cells 2.3-fold. The stimulatory effect of EGF, TGF-a and the combination of E2 and P on DNA replication in the uterine stromal cells was repressed by RG-13022 (10(-5) M, the inhibitor of the EGF receptor tyrosine kinase). RT-PCR analysis of EGF-receptor-, TGF-alpha, and EGF-mRNA was carried,out in the cultured uterine stromal cells, and revealed the expression of those mRNAs. These data supported the hypothesis that uterine endometrial stromal growth induced by sex steroids required the EGF family of ligands such as EGF and TGF-alpha, both produced in the stromal cells, acting for DNA synthesis through EGF receptors.
机译：子宫内膜产生的生长因子被认为与雌二醇-17β（E-2）和孕酮（P）诱导的小鼠子宫基质细胞的增殖有关。在无血清培养物中研究了表皮生长因子（EGF）和转化生长因子-α（TGF-α）（一种与EGF相关的生长因子）对小鼠子宫基质细胞增殖的影响。通过MTT测定法测量子宫基质细胞的生长。发现EGF以剂量依赖性方式增加子宫基质细胞的数量。使用溴脱氧尿苷（BrdU）标记的细胞的免疫细胞化学检测来研究DNA复制细胞。 EGF和TGF-α以剂量依赖性方式增加BrdU-Iabeled细胞的百分比。 E-2（10（-9）M）和P（10（-7）M）的组合给药2天可使BrdU-Iabeled细胞的百分比增加2.3倍。 RG-13022（10（-5）M，EGF受体酪氨酸激酶的抑制剂）抑制了EGF，TGF-α以及E2和P的结合对子宫基质细胞DNA复制的刺激作用。在培养的子宫基质细胞中进行了EGF-受体，TGF-α和EGF-mRNA的RT-PCR分析，并揭示了这些mRNA的表达。这些数据支持以下假设：性类固醇诱导子宫内膜基质生长需要EGF族配体，例如EGF和TGF-α，它们都在基质细胞中产生，通过EGF受体作用于DNA合成。

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