...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Zoological Science >Five different types of putative GnRH receptor gene are expressed in the brain of masu salmon (Oncorhynchus masou)
【24h】

Five different types of putative GnRH receptor gene are expressed in the brain of masu salmon (Oncorhynchus masou)

机译：五种类型的推定的GnRH受体基因在鲑鱼的大脑中表达（Oncorhynchus masou）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recent studies have shown that there are multiple genes encoding gonadotropin-releasing hormone receptor (GnRH-R) in single species. In salmonids, however, only a single gene has been identified in the rainbow trout. We therefore isolated partial cDNAs from the brain and the pituitary of masu salmon Oncorhynchus masou by reverse transcription-polymerase chain reaction and 5'-rapid amplification of cDNA ends, using primers corresponding to conserved transmembrane domains (TMs). Five different partial cDNAs were isolated from an individual and termed as msGnRH-R1, R2, R3, R4 and R5. They are divided into two groups, msGnRH-R1, R2, R3 and msGnRH-R4, R5. Two groups share 59-71% nucleotide sequence identities. Phylogenetic analysis showed that the former group is closely related to the goldfish GnRH-R GfA, and the latter to GfB. All five msGnRH-R genes were expressed in the brain and msGnRH-R1, R3 and R5 were expressed in the pituitary. In addition, we found mRNA for msGnRH-R1 in the kidney and ovary, and R2 in the ovary, whereas msGnRH-R5 gene was widely expressed in the muscle, heart, kidney and testis. Differences in the expression of msGnRH-R genes between maturing and spawning fish were observed in the brain and pituitary, except for the constantly expressed msGnRH-R5. A splicing variant of msGnRH-R1 mRNA that is capable of generating a truncated GnRH-R that consists of 5TMs was also expressed in the brain, pituitary and kidney. These results indicate that five different types of putative GnRH-R gene are present and expressed in the brain of masu salmon.
机译：最近的研究表明，在单个物种中有多个编码促性腺激素释放激素受体（GnRH-R）的基因。然而，在鲑鱼中，虹鳟中仅鉴定出一个基因。因此，我们使用对应于保守跨膜结构域（TMs）的引物，通过逆转录聚合酶链反应和cDNA末端的5'-快速扩增，从马苏鲑Oncorhynchus masou的大脑和垂体中分离了部分cDNA。从个体中分离出五个不同的部分cDNA，分别称为msGnRH-R1，R2，R3，R4和R5。它们分为两组，分别为msGnRH-R1，R2，R3和msGnRH-R4，R5。两组共有59-71％的核苷酸序列同一性。系统发育分析表明，前者与金鱼GnRH-R GfA密切相关，后者与GfB密切相关。所有五个msGnRH-R基因在大脑中表达，而msGnRH-R1，R3和R5在垂体中表达。此外，我们在肾脏和卵巢中发现了msGnRH-R1的mRNA，在卵巢中发现了R2的mRNA，而msGnRH-R5基因则在肌肉，心脏，肾脏和睾丸中广泛表达。除了不断表达的msGnRH-R5外，在大脑和垂体中还观察到了成熟鱼和产卵鱼之间msGnRH-R基因表达的差异。还能够在脑，垂体和肾脏中表达msGnRH-R1 mRNA的剪接变体，该变体能够产生由5个TM组成的截短的GnRH-R。这些结果表明，在马苏鲑的脑中存在并表达了五种不同类型的假定的GnRH-R基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号