Fluorescent in situ hybridization to ascidian chromosomes

Shoguchi B; Ikuta T; Yoshizaki F; Satou Y; Satoh N; Asano K; Saiga

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Fluorescent in situ hybridization to ascidian chromosomes

机译：荧光原位杂交至海鞘染色体

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The draft genome of the ascidian Ciona intestinalis has been sequenced. Mapping of the genome sequence to the Ciona 14 haploid chromosomes is essential for future studies of the genome-wide control of gene expression in this basal chordate. Here we describe an efficient protocol for fluorescent in situ hybridization for mapping genes to the Ciona chromosomes. We demonstrate how the locations of two BAC clones can be mapped relative to each other. We also show that this method is efficient for coupling two so-far independent scaffolds into one longer scaffold when two BAC clones represent sequences located at either end of the two scaffolds.

机译：已对海鞘Ciona intestinalis的基因组草图进行了测序。将基因组序列映射到Ciona 14单倍体染色体对于将来研究该基体碳酸盐中基因表达的全基因组控制至关重要。在这里，我们描述了一种有效的荧光原位杂交方案，用于将基因定位到Ciona染色体。我们演示了如何可以相对于彼此映射两个BAC克隆的位置。我们还表明，当两个BAC克隆代表位于两个支架任一末端的序列时，该方法可有效地将两个迄今为止非常独立的支架偶联到一个更长的支架中。

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来源
《Zoological Science》 |2004年第2期|共5页
作者
Shoguchi B; Ikuta T; Yoshizaki F; Satou Y; Satoh N; Asano K; Saiga;
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正文语种 eng
中图分类动物学;
关键词
Ascidians; Fluorescent in situ hybridization; Mapping of genes; Developmentally relevant genes; Ciona-intestinalis; Genomewide survey; Transcription factors; Expression profiles; Chordate.;

机译：海鞘;荧光原位杂交;基因作图;发育相关基因;肠虫;全基因组调查;转录因子;表达谱;果核。;

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1. Fluorescent in situ hybridization to ascidian chromosomes [J] . Shoguchi B, Ikuta T, Yoshizaki F, Zoological Science . 2004,第2期

机译：荧光原位杂交至海鞘染色体
2. Rapid positive confirmation of mosaicism for a small supernumerary marker chromosome as r(8) by interphase fluorescence in situ hybridization, quantitative fluorescent polymerase chain reaction, and array comparative genomic hybridization on uncultured amniocytes in a pregnancy with fetal pyelectasis [J] . ChenC.-P., ChangS.-D., SuY.-N., Taiwanese journal of obstetrics and gynecology . 2012,第3期

机译：通过妊娠期未合并培养的羊膜细胞间期荧光原位杂交，定量荧光聚合酶链反应和阵列比较基因组杂交技术，快速确定阳性的小数目标记染色体r（8）的镶嵌性
3. Rapid positive confirmation of mosaicism for a small supernumerary marker chromosome as r(8) by interphase fluorescence in situ hybridization, quantitative fluorescent polymerase chain reaction, and array comparative genomic hybridization on uncultured amniocytes in a pregnancy with fetal pyelectasis [J] . Chih-Ping Chen, Shuenn-Dyh Chang, Yi-Ning Su, Taiwanese journal of obstetrics and gynecology . 2012,第3期

机译：通过相间荧光原位杂交，定量荧光聚合酶链反应和阵列比较基因组杂交对未培养的羊水中的小数量标记染色体r（8）进行镶嵌的快速阳性确认。妊娠合并胎儿眼睑扩张
4. Recent advances in image analysis of chromosomes subjected to HG-banding and fluorescent in situ hybridization (FISH) [C] . Novak, A., Jovanovic, . 1998

机译：HG带和荧光原位杂交（FISH）染色体图像分析的最新进展
5. Improved detection of chromosomal abnormalities in low grade or emerging myelodysplasia utilizing interphase fluorescent in situ hybridization [D] . Matthews, Michael S. 2008

机译：利用相间荧光原位杂交技术改进对低度或新兴骨髓发育异常的染色体异常的检测
6. Identification of small marker chromosomes using microarray comparative genomic hybridization and multicolor fluorescent in situ hybridization [O] . Woori Jang, Hyojin Chae, Jiyeon Kim, 2015

机译：使用微阵列比较基因组杂交和多色荧光原位杂交鉴定小标记染色体
7. Identification of small marker chromosomes using microarray comparative genomic hybridization and multicolor fluorescent in situ hybridization [O] . Woori Jang, Hyojin Chae, Jiyeon Kim, 2016

机译：使用微阵列比较基因组杂交和多色荧光原位杂交鉴定小标记染色体

Fluorescent in situ hybridization to ascidian chromosomes

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