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An evidence of stromal cell populations functionally linked with epithelial cell populations in the mouse oviduct

机译:小鼠输卵管中基质细胞群与上皮细胞群功能相关的证据

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摘要

The oviductal epithelium consists of two major cell populations, secretory cells and cilial cells. In a previous report, we established clonal cell lines from the epithelium and stroma of an oviduct which allowed us to analyze stromal contribution to epithelial functions. Three stromal cell lines were cocultured in separated apparatus with 3 epithelial cell lines, respectively. Two stromal cell lines preferentially stimulated mogp-1 expression on secretory cells and the stimulation was additive with estrogen. The lines had no effect on cilial cells. One stromal cell line preferentially stimulated foxj1 expression on cilial cells and the stimulation relieved suppression by estrogen. The line had no effect on secretory cells. Experiments with conditioned media of the stromal cells confirmed the results of co-culture experiments, suggesting that the oviductal stroma contains multiple cell populations preferentially regulating or modulating specific cell populations of the epithelium via diffusible factors.
机译:输卵管上皮由两个主要细胞群组成,即分泌细胞和纤毛细胞。在以前的报告中,我们从输卵管的上皮和间质建立了克隆细胞系,这使我们能够分析基质对上皮功能的贡献。将三种基质细胞系分别在分离的装置中与3种上皮细胞系共培养。两个基质细胞系优先刺激分泌细胞上的mogp-1表达,并且刺激与雌激素相加。该品系对纤毛细胞没有影响。一种基质细胞系优先刺激foxj1在纤毛细胞上的表达,刺激减轻了雌激素的抑制作用。该品系对分泌细胞没有影响。用基质细胞的条件培养基进行的实验证实了共培养实验的结果,表明输卵管基质含有多个细胞群体,这些细胞群体优先通过扩散因子调节或调节上皮的特定细胞群体。

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