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首页> 外文期刊>Zoological Science >Molecular cloning of cDNA encoding a 20S proteasome alpha 2 subunit fromgoldfish (Carassius auratus) and its expression analysis
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Molecular cloning of cDNA encoding a 20S proteasome alpha 2 subunit fromgoldfish (Carassius auratus) and its expression analysis

机译：金鱼（Car鱼）20S蛋白酶体α2亚基编码cDNA的分子克隆及其表达分析

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摘要

Proteasomes are large, multisubunit particles that act as the proteolytic machinery for most regulated intracellular protein breakdown in eukaryotic cells. The core proteinase of this complex, the 20S proteasome, is comprised of four stacked rings with seven subunits each. The outer two rings are made up of seven, distinct alpha-type subunits, while the two inner rings are composed of seven, different beta-type subunits. Here we present the cloning, sequencing and expression analysis of Carassius auratus, alpha 2_ca, which encodes one of the proteasome alpha subunits from goldfish ovary. The cloned cDNA is 838 bp long and encodes 234 amino acids. The deduced amino acid sequence is highly homologous to alpha 2 subunits from other vertebrates. The expression of mRNA for alpha 2_ca occurs at very high levels in ovary and muscle and moderately high levels in testis, brain and gill. It was also shown that protein content was different from mRNA expression levels.
机译：蛋白酶体是大的，多亚基的颗粒，可作为真核细胞中大多数受调节的细胞内蛋白质分解的蛋白水解机制。该复合物的核心蛋白酶20S蛋白酶体由四个堆叠的环组成，每个环具有七个亚基。外两个环由七个不同的α型亚基组成，而两个内环由七个不同的β型亚基组成。在这里，我们介绍of鱼α2_ca的克隆，测序和表达分析，它编码来自金鱼卵巢的蛋白酶体α亚基之一。克隆的cDNA长838 bp，编码234个氨基酸。推导的氨基酸序列与其他脊椎动物的α2亚基高度同源。 alpha 2_ca的mRNA表达在卵巢和肌肉中的表达很高，而在睾丸，大脑和腮中的表达也中等。还显示蛋白质含量与mRNA表达水平不同。

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