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首页> 外文期刊>Zoological Science >A circadian neuropeptide PDF in the honeybee, Apis mellifera: cDNA cloning and expression of mRNA.
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A circadian neuropeptide PDF in the honeybee, Apis mellifera: cDNA cloning and expression of mRNA.

机译：蜜蜂中的昼夜节律神经肽PDF Apis mellifera ：cDNA克隆和mRNA表达。

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摘要

Pigment-dispersing factor (PDF) is a pacemaker hormone regulating the locomotor rhythm in insects. In the present study, we cloned the cDNAs encoding the Apis PDF precursor protein, and found that there are at least seven different pdf mRNAs yielded by an alternative splicing site and five alternative polyadenylation sites in the 5'UTR and 3'UTR regions. The amino acid sequence of Apis PDF peptide has a characteristic novel amino acid residue, aspargine (Asn), at position 17. Quantitative real-time PCR of total and 5'UTR insertion-type pdf mRNAs revealed, for the first time, that the expression levels change in a circadian manner with a distinct trough at the beginning of night in LD conditions, and at the subjective night under DD conditions. In contrast, the expression level of 5'UTR deletion-type pdf mRNAs was about half of that of the insertion type, and the expression profile failed to show a circadian rhythm. As the expression profile of the total pdf mRNA exhibited a circadian rhythm, transcription regulated at the promoter region was supposed to be controlled by some of the clock components. Whole mount in situ hybridization revealed that 14 lateral neurons at the frontal margin of the optic lobe express these mRNA isoforms. PDF expressing cells examined with a newly produced antibody raised against Apis PDF were also found to have a dense supply of axon terminals in the optic lobes and the central brain.Digital Object Identifier http://dx.doi.org/10.2108/zsj.28.897
机译：色素分散因子（PDF）是起搏器激素，可调节昆虫的运动节奏。在本研究中，我们克隆了编码 Apis PDF前体蛋白的cDNA，发现至少有7种不同的 pdf mRNA是由一个替代剪接位点和五个替代5'UTR和3'UTR区域的聚腺苷酸化位点。 Apis PDF肽的氨基酸序列在第17位具有特征性的新氨基酸残基，天冬酰胺（Asn）。定量实时PCR的5'UTR插入型 pdf mRNA首次揭示，在LD条件下的夜晚开始和DD条件下的主观夜晚，表达水平以昼夜节律的方式变化，并有一个明显的低谷。相比之下，5'UTR缺失型 pdf mRNA的表达水平约为插入类型的一半，并且表达谱未能显示出昼夜节律。由于全部 pdf mRNA的表达谱均具有昼夜节律，因此推测在启动子区域调节的转录受某些时钟成分控制。整个安装原位杂交表明，在视神经叶的前缘有14个外侧神经元表达了这些mRNA异构体。还发现用新产生的针对 Apis PDF的抗体检查的PDF表达细胞在视神经叶和中枢脑中有大量轴突末端供体。数字对象标识符http：//dx.doi .org / 10.2108 / zsj.28.897

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