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首页> 外文期刊>Zoological Science >The ter/ter gonadal somatic cells cause apoptosis in ter/ter primordialgerm cells (PGCs) with normal survivability and proliferation ability inthe mouse: Evidence from PGC-Somatic cell 'exchange-co-culture'
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The ter/ter gonadal somatic cells cause apoptosis in ter/ter primordialgerm cells (PGCs) with normal survivability and proliferation ability inthe mouse: Evidence from PGC-Somatic cell 'exchange-co-culture'

机译：ter / ter性腺体细胞在小鼠中引起具有正常生存能力和增殖能力的ter / ter原始生殖细胞（PGC）凋亡：PGC-体细胞“交换-共培养”的证据

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The ter (teratoma, chromosome 18) mutation causes a deficiency in primordial germ cells (PGCs) of ter/ter embryos from ter congenic mouse strains at around 8.0 days post coitum (dpc). Our previous studies indicated that in vivo, apoptosis of PGCs was caused by ter/ter gonadal somatic cells. To examine survival and proliferation in ter/ter PGCs and the deficiency caused by ter/ter gonadal somatic cells in vitro, we performed "exchange-co-culture" of PGCs and gonadal somatic cells by combining different ter-genotypes, on SI/SI4-m220 feeder cells. The number of PGCs after 3 days culture of 9.5 dpc ter/ter PGCs with +/+ 12.5 dpc gonadal somatic cells increased similar to that of +/ter or +/+ PGCs. The numbers of PGCs after 12 hr culture of +/+ and ter/ter 11.5 dpc PGCs with 11.5 dpc ter/ter gonadal somatic cells decreased significantly when compared to those cultured with +/+ somatic cells. PGCs preferred the WT1-positive gonadal somatic cells, Sertoli cells, to the feeder cells. Both TUNEL and BrdU assays showed that ter/ter somatic cells induced apoptosis but were independent of DNA synthesis in PGCs "exchange-co-cultured". Through these results, we demonstrated for the first time that in vitro ter/ter PGCs showed survival and proliferation activities in response to the gonadal somatic cells and that ter/ter gonadal somatic cells caused apoptosis in PGCs through cell-cell contact.
机译：ter（畸胎瘤，第18号染色体）突变会导致在coitum（dpc）约8.0天后，来自ter同系小鼠品系的ter / ter胚胎的ter / ter胚胎的原始生殖细胞（PGC）缺乏。我们以前的研究表明，在体内，PGC的凋亡是由性腺/叔体细胞引起的。为了检查ter / ter PGCs的存活和增殖以及由ter / ter的性腺体细胞引起的体外缺乏，我们通过在SI / SI4上结合不同的ter-gentypes对PGC和性腺的体细胞进行“交换-共培养” -m220饲养细胞。用+ / + 12.5 dpc性腺体细胞培养9.5 dpc ter / ter PGC 3天后，PGC的数量增加，与+ / ter或+ / + PGC相似。与用+ / +体细胞培养的相比，用11.5 dpc ter / ter性腺的体细胞培养+ / +和ter / ter 11.5 dpc PGC的12小时后，PGC的数量显着减少。 PGC更喜欢WT1阳性的性腺体细胞，Sertoli细胞，而不是饲养细胞。 TUNEL和BrdU分析均显示，ter / ter体细胞诱导凋亡，但与“交换-共培养”的PGC中的DNA合成无关。通过这些结果，我们首次证明了体外ter / ter PGCs表现出对性腺体细胞应答的存活和增殖活性，而ter / ter性腺体细胞通过细胞间接触引起了PGCs的凋亡。

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