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首页> 外文期刊>Zoological Science >Shrinkage and expansion of blastocoel affect the degree of invagination in sea urchin embryos
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Shrinkage and expansion of blastocoel affect the degree of invagination in sea urchin embryos

机译：囊胚腔的收缩和膨胀影响海胆胚胎的内陷程度

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By immersing sea urchin embryos in seawater containing various concentrations of sucrose, we found that the level of osmotic pressure of blastocoel changed during gastrulation; the level was low around the onset of gastrulation and higher during secondary invagination. To learn how such changes in osmotic pressure related to invagination processes, embryos were shrunken or expanded by means of sucrose treatments, and the degree of invagination was monitored using Nile blue staining. The method elucidated that the cells invaginated during primary invagination occupied only top one third of archenteron at the end of secondary invagination, and that the cells constituting intestine and the posterior half of stomach were recruited into the archenteron after secondary invagination. When embryos were expanded, the degree of invagination was remarkably decreased, indicating that a higher level of expanding force hindered the fulfillment of invagination. On the other hand, shrinkage of blastocoel during secondary invagination increased the degree of invagination. Cell tracing experiments showed that the change in the degree of invagination estimated with Nile blue staining was accompanied with the change in the number of cells incorporated into the archenteron. When embryos were expanded, only the descendants of veg2 constituted the gut rudiment, while a considerable amount of the veg1 descendants were recruited into archenteron when embryos were shrunken. Obtained results suggest that change in the level of osmotic pressure of blastocoel would be necessary for the progress of gastrulation.
机译：通过将海胆胚胎浸入含有各种浓度蔗糖的海水中，我们发现胚芽过程中囊胚腔的渗透压水平发生了变化。胃蠕动开始时水平较低，继发性内陷时水平较高。为了了解渗透压的这种变化如何与内陷过程相关，通过蔗糖处理使胚胎收缩或膨胀，并使用尼罗蓝染色监测内陷程度。该方法阐明了在初次内陷过程中所侵袭的细胞在二次内陷过程结束时仅占据了原肠的上三分之一，并且在第二次内陷后，构成肠和胃后半部的细胞被募集到原肠中。当胚胎扩张时，内陷程度明显降低，这表明较高水平的扩张力阻碍了内陷的完成。另一方面，在二次内陷期间囊胚腔的收缩增加了内陷的程度。细胞示踪实验表明，用尼罗蓝染色估计的内陷程度的变化伴随着进入原肠的细胞数量的变化。当胚胎扩张时，只有veg2的后代构成了肠的雏形，而当胚胎收缩时，大量的veg1的后代被召集到了肠ent中。获得的结果表明，改变囊胚的渗透压水平对于进行胃形成是必要的。

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