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首页> 外文期刊>Zoological Science >Zili Antagonizes Bmp Signaling to Regulate Dorsal-ventral Patterning during Zebrafish Early Embryogenesis
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Zili Antagonizes Bmp Signaling to Regulate Dorsal-ventral Patterning during Zebrafish Early Embryogenesis

机译：Zili拮抗Bmp信号以调节斑马鱼早期胚胎发生过程中的背腹模式。

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摘要

Bone morphogenetic protein (Bmp) signaling plays a pivotal role in dorsal-ventral (DV) patterning in vertebrate embryos. Piwi proteins are required for germline and stem cell development. Our previous study demonstrated that Zili, zebrafish Piwil2, inhibits transforming growth factor (TGF)-beta signaling by interacting with Smad4, suggesting a role for zili in Bmp signaling. In the present study, zili-MO or zili mRNA was microinjected into one-cell embryos to knock down or elevate the expression of zili to study the role of zili during early zebrafish embryogenesis. Knockdown of zili inhibited the expression of dorsal marker genes, and enhanced that of ventral marker genes. In contrast, overexpression of zili promoted expression of dorsal marker genes, while it inhibited ventral marker genes. These results suggest that zili regulates DV patterning. The influence of zili on the Bmp pathway was further explored. Knockdown of zili resulted in higher expression levels of bmp2b, and bmp4, the Bmp signaling ligands, and reduced expression of chordin (chd), noggin (nog1), and follistatin (fst), which encode BMP antagonists. Meanwhile, overexpression of zili produced opposite effects. In conclusion, our results indicate that zili regulates dorsal-ventral patterning by antagonizing Bmp signaling during early embryogenesis in zebrafish.
机译：骨形态发生蛋白（Bmp）信号传导在脊椎动物胚胎的背腹（DV）模式中起关键作用。 Piwi蛋白是种系和干细胞发育所必需的。我们以前的研究表明，Zili（斑马鱼Piwil2）通过与Smad4相互作用抑制转化生长因子（TGF）-β信号传导，表明zili在Bmp信号传导中发挥了作用。在本研究中，将zili-MO或zili mRNA微注射到单细胞胚胎中以敲低或提高zili的表达，以研究zili在斑马鱼早期胚胎发生中的作用。抑制zili可抑制背侧标记基因的表达，并增强腹侧标记基因的表达。相反，zili的过表达促进了背侧标记基因的表达，而抑制了腹侧标记基因。这些结果表明自力调节DV模式。进一步探讨了zili对Bmp途径的影响。敲除zili会导致bmp2b和bmp4（Bmp信号配体）的表达水平更高，并降低编码BMP拮抗剂的chordin（chd），noggin（nog1）和卵泡抑素（fst）的表达。同时，zili的过表达产生相反的作用。总之，我们的结果表明，zili通过在斑马鱼的早期胚胎发生过程中拮抗Bmp信号传导来调节背腹模式。

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