• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Zoological Science >Amitotic Division of the Macronucleus in Tetrahymena thermophila: DNA Distribution by Genomic Unit
【24h】

Amitotic Division of the Macronucleus in Tetrahymena thermophila: DNA Distribution by Genomic Unit

机译：嗜热四膜膜大细胞核的有丝分裂区：按基因组单位的DNA分布

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The macronucleus of the ciliate Tetrahymena cell contains euchromatin and numerous heterochromatins called chromatin bodies. During cell division, a chromatin aggregate larger than chromatin body appears in the macronucleus. We observed chromatin aggregates in the dividing macronucleus in a living T. thermophila cell, and found that these were globular in morphology and homogeneous in size. To observe globular chromatin clearly, optimal conditions for making it compact were studied. Addition of Mg ion, benomyl and oryzalin, microtubule inhibitors, to cell suspension was effective. Globular chromatin appeared when the micronuclear anaphase began at the cell cortex, and disappeared long after cell separation. Using living cells with a small macronucleus at early log phase, we counted the number of globular chromatin per nucleus and measured the DNA content of globular chromatin in the macronucleus which was stained with Hoechst 33342 by using ImageJ. The number of globular chromatin per nucleus was reduced by half after division, indicating the globular chromatin is a distribution unit of DNA. A globular chromatin contained similar DNA content as that of the macronuclear genome. We developed methods for inducing and isolating a cell with an extremely small macronucleus with a DNA amount of one globular chromatin. These cells grew, divided, and give clones, suggesting that the macronuclear genome is not dispersed within the macronucleus and the globular chromatin may be a macronuclear genome. We named this globular chromatin "macronuclear genome unit" (MGU).
机译：纤毛四膜虫细胞的大核含有常染色质和许多称为染色质体的异染色质。在细胞分裂期间，大核中出现了比染色质体大的染色质聚集体。我们观察到在活嗜热衣原体细胞中分裂的大核中的染色质聚集体，发现它们的形态呈球形，大小均一。为了清楚地观察球状染色质，研究了使其紧凑的最佳条件。在细胞悬浮液中加入镁离子，苯菌灵和米扎林微管抑制剂是有效的。当微核后期从细胞皮层开始时出现球状染色质，在细胞分离后很长时间消失。使用早期对数期具有小大核的活细胞，我们计算每个核的球状染色质的数目，并测量大核中球状染色质的DNA含量，并通过ImageJ用Hoechst 33342染色。分裂后，每个核的球状染色质数量减少了一半，这表明球状染色质是DNA的分布单位。球状染色质包含与大核基因组相似的DNA含量。我们开发了诱导和分离细胞的方法，该细胞具有一个非常小的大核，DNA量为一个球状染色质。这些细胞生长，分裂并产生克隆，表明大核基因组没有分散在大核内，球状染色质可能是大核基因组。我们将该球状染色质命名为“大核基因组单位”（MGU）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号