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Comparative Cytogenetics Among Populations of Hollandichthys multifasciatus (Teleostei: Characidae)

机译:凤尾鱼(Teleostei:Characidae)种群之间的比较细胞遗传学。

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摘要

Two populations of Hollandichthys multifasciatus from headwaters of adjacent river coastal basins in Parana state, southern Brazil, were cytogenetically studied in order to evaluate intraspecific divergences. Both populations presented 2n = 50 chromosomes, divided into 14m + 18sm + 18st in the sample from Antonina and 14m + 20sm + 16st for the population from Guaraquecaba. Analyses of active nucleolar organizer regions (Ag-NORs) and fluorescent in situ hybridization (FISH) with 18S rDNA probes revealed a single metacentric pair bearing marks at proximal positions on the short arms. The location of the 5S rDNA and GC-rich sites showed chromosomal divergence between both populations. Therefore, several population markers were detected, reflecting a population differentiation, possibly driven by the formation of Paranagua Bay and Serra do Mar mountain range. Since the existence of a species complex in H. multifasciatus has been previously characterized by morphological, molecular, and karyotype population differentiation, this species needs to be taxonomically studied in detail.
机译:对巴西南部巴拉那州相邻河流沿岸水源的两个河豚种群进行了细胞遗传学研究,以评估种内差异。两种种群均呈现2n = 50条染色体,从Antonina的样本中分为14m + 18sm + 18st,而从瓜拉奎卡巴的种群中分为14m + 20sm + 16st。用18S rDNA探针对活性核仁组织区(Ag-NORs)和荧光原位杂交(FISH)进行分析,发现在短臂的近端位置有单个亚中心对标记。 5S rDNA和富含GC的位点的位置表明两个种群之间的染色体差异。因此,检测到一些人口标志,反映了人口分化,这可能是由巴拉那瓜湾和塞拉杜马尔山脉的形成驱动的。由于以前已通过形态,分子和核型种群分化来表征多发带状疱疹物种复合体的存在,因此需要对该物种进行详细的分类学研究。

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