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首页> 外文期刊>Zoological Science >DNA Barcoding as a Tool for Elucidating Species Delineationin Wide-ranging Species as Illustrated by Owls (Tytonidae and Strigidae)
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DNA Barcoding as a Tool for Elucidating Species Delineationin Wide-ranging Species as Illustrated by Owls (Tytonidae and Strigidae)

机译：DNA条形码作为阐明种类的工具，例如猫头鹰（Tytonidae和Strigidae）

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The mitochondrial cytochrome c-oxidase subunit I (cox1) can serve as a fast and accurate marker for the identification of animal species, and for the discovery of new species across the tree of life. Distinguishing species using this universal molecular marker, a technique known as DNA barcoding, relies on the identifying the gap between intra-and interspecific divergence. One of the difficulties could be wide-ranging, cosmopolitan species that show large amounts of morphological variation. The barn owl Tyto alba is a case in point. It occurs worldwide and varies morphologically, leading to the recognition of many subspecies or, more recently, species. We analysed data from the cox1 gene for 31 individuals of seven subspecies, and compared this with 214 sequences from 29 other owl species. Phylogenetic analysis of the T. alba samples gives very strong support for an Old World alba-clade (three subspecies) and a New World furcata-clade (four subspecies) that are genetically equidistant. The amount of intraspecific variation within each of these clades ranges from 0.66-0.99%, but variation among these clades ranges from 5.33-6.20%. Combined these data suggest that barn owl of the Old World is indeed best considered a separate species different from that of the New World. For combined dataset, sample size of owl species (n between 1 and 21 sequences) increased with geographic range size but we did not find significant relationships between interspecific divergence and sample size or between interspecific divergence and geographic range. For 21/24 species of owls with sample sizes of n >= 4 the maximum interspecific divergences was <= 3.00%. However, similar to those found in barn owls, the largest amount of divergence (3.23-4.09%) was present in two other wide-ranging species (Strix nebulosa and Aegolius funereus) raising the possibility of multiple species in other wide-ranging owls as well.
机译：线粒体细胞色素c-氧化酶亚基I（cox1）可以作为快速准确的标记物，用于识别动物物种，以及在整个生命树中发现新物种。使用这种通用分子标记物（一种称为DNA条形码）的技术来区分物种依赖于鉴定种内和种间差异之间的间隙。困难之一可能是范围广泛的大都会物种，它们表现出大量的形态变异。仓owl Tyto alba就是一个很好的例子。它在全球范围内发生，并且在形态上有所不同，从而导致人们认识了许多亚种，或者最近才发现了物种。我们分析了来自cox1基因的七个亚种的31个个体的数据，并将其与其他29种猫头鹰的214个序列进行了比较。对T. alba样品的系统发育分析为遗传等距的旧世界alba-clade（三个亚种）和New World furcata-clade（四个亚种）提供了非常有力的支持。这些进化枝中的每个进化枝内的种内变异量为0.66-0.99％，但是这些进化枝之间的变异为5.33-6.20％。综合这些数据表明，最好将旧大陆的仓视为与新大陆不同的单独物种。对于组合数据集，猫头鹰物种的样本大小（n在1至21个序列之间）随地理范围大小而增加，但我们并未发现种间差异与样本大小之间或种间差异与地理范围之间的显着关系。对于n / = 4的样本大小的21/24种猫头鹰，最大种间差异为<= 3.00％。但是，与在谷仓猫头鹰中发现的相似，在其他两个大范围物种中（Strix nebulosa和Aegolius funereus）存在最大的差异（3.23-4.09％），这增加了在其他大范围猫头鹰中存在多个物种的可能性，例如好。

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