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首页> 外文期刊>Zoological Science >Molecular Cloning and Transcript Expression of Genes Encoding Two Types of Lipoprotein Lipase in the Ovary of Cutthroat Trout, Oncorhynchus clarki
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Molecular Cloning and Transcript Expression of Genes Encoding Two Types of Lipoprotein Lipase in the Ovary of Cutthroat Trout, Oncorhynchus clarki

机译：Cut鳟鳟鱼卵巢中两种脂蛋白脂酶编码基因的分子克隆和转录表达

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摘要

Large amounts of neutral lipids (NLs) are stored as lipid droplets in the ooplasm of fish oocytes, providing an essential energy resource for developing embryos and larvae. However, little is known about the origin of such lipids or about mechanisms underlying their uptake and accumulation in oocytes. We have proposed a model for this lipidation of teleost oocytes, as follows: very low density lipoprotein (Vldl) is metabolized by lipoprotein lipase (Lpl) outside and/or inside of the oocyte and the resulting fatty acids (FAs) are then utilized for de novo biosynthesis of NLs. As a first step toward verification of this model, cDNAs for genes encoding two types of Lpl, lpl1 and lpl2, were cloned from the ovary of cutthroat trout, Oncorhynchus clarki. Examination of Lpl polypeptide sequences deduced from the cDNAs revealed features similar to LPLs/Lpls in other species, including several conserved structural and functional domains. Both types of lpl mRNA were highly expressed in lipid storage tissues (e. g., adipose tissue, muscle, and ovary) and were predominantly expressed in the granulosa cells of ovarian follicles. Ovarian lpl1 mRNA levels showed a remarkable peak in April (early oocyte lipid droplet stage) and then decreased to low values sustained until November (mid-vitellogenesis), after which time a small peak in lpl1 gene expression was observed in December (late vitellogenesis). The mRNA levels of lpl2 also were elevated in April and were highest in June (late lipid droplet stage), but did not show other pronounced changes. These results suggest that, in the cutthroat trout, Vldl is metabolized by the action of Lpls in the granulosa cell layer to generate free FAs for uptake and biosynthesis of neutral lipids by growing oocytes.
机译：大量中性脂质（NLs）以脂质滴的形式储存在鱼卵母细胞的卵母液中，为发育胚胎和幼虫提供了必要的能源。然而，关于这种脂质的起源或它们在卵母细胞中摄取和积累的机制尚不清楚。我们提出了一种用于硬骨质卵母细胞脂质化的模型，如下所示：卵母细胞外部和/或内部的脂蛋白脂肪酶（Lpl）代谢非常低密度的脂蛋白（Vldl），然后将所得脂肪酸（FAs）用于从头开始生物合成NLs。作为验证该模型的第一步，从尖吻鳟鳟鱼（Oncorhynchus clarki）的卵巢中克隆了编码两种类型的Lpl（lpl1和lpl2）的基因的cDNA。从cDNA推论的Lpl多肽序列的检查揭示了与其他物种中的LPL / Lpl类似的特征，包括几个保守的结构和功能结构域。两种类型的lpl mRNA在脂质存储组织（例如，脂肪组织，肌肉和卵巢）中高表达，并且主要在卵巢卵泡的颗粒细胞中表达。卵巢lpl1 mRNA水平在4月（卵母细胞脂质液滴早期）出现一个显着的峰值，然后下降到持续至11月的低值（中卵形成），此后在12月观察到lpl1基因表达的一个小峰值（后期卵黄形成）。 。 lpl2的mRNA水平在4月也升高并且在6月最高（脂滴后期），但是没有显示其他明显的变化。这些结果表明，在凶猛的鳟鱼中，Vldl通过颗粒细胞层中Lpls的作用而代谢，从而生成游离的FAs，以通过卵母细胞的生长来摄取和生物合成中性脂质。

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