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首页> 外文期刊>Zoological Science >Relationship Between Two Androgenetic Clam Species, Corbicula leana and Corbicula fluminea, Inferred from Mitochondrial Cytochrome b and Nuclear 28S rRNA Markers
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Relationship Between Two Androgenetic Clam Species, Corbicula leana and Corbicula fluminea, Inferred from Mitochondrial Cytochrome b and Nuclear 28S rRNA Markers

机译：从线粒体细胞色素b和核28S rRNA标记推断出两种雄性蛤lam物种Corbicula leana和Corbicula fluminea之间的关系。

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摘要

Two shell color types, yellow (type I) and brown (type II), of hermaphrodite Corbicula fluminea clams from Ritto, Shiga Prefecture, Japan, are sympatric with both male and hermaphrodite Corbicula leana. In the present study, the mitochondrial DNA (mtDNA) cytochrome b and nuclear 28S rRNA genes of C. fluminea were sequenced to construct a haplotype network in order to investigate the genetic relationship with C. leana. Ninety C. fluminea samples revealed only two cytb haplotypes; the majority (97.8%) were CB7, while the remainder were CB1. In C. leana, only CB1 was detected in hermaphrodites, but both CB1 and CB7 were detected in males. Nuclear 28S rRNA haplotypes of C. fluminea type I individuals were divergent from those of hermaphrodite C. leana. However, C. fluminea type I clams shared haplotypes with male C. leana individuals, whereas C. fluminea type II individuals shared haplotypes with both hermaphrodite and male C. leana samples. These results suggest that it may be difficult to define a clear genetic border between these species.
机译：来自日本滋贺县里托的雌雄同株Corbicula fluminea蛤的两种壳颜色，黄色（I型）和棕色（II型）与雄性和雌雄同体的Corbicula leana同卵。在本研究中，对线粒体线粒体DNA（mtDNA）细胞色素b和氟链霉菌的核28S rRNA基因进行测序，以构建单倍型网络，以研究与C. leana的遗传关系。九十个C. fluminea样本显示只有两种cytb单倍型;大部分（97.8％）为CB7，其余为CB1。在C. leana中，雌雄同体中仅检测到CB1，而雄性中同时检测到CB1和CB7。我的C. fluminea I型个体的核28S rRNA单倍型与雌雄同体的C. leana不同。但是，第一类C. fluminea蛤与雄性C. leana个体共享单倍型，而第二种C. fluminea II型个体与雌雄同体和雄性C. leana样本共享单倍型。这些结果表明，可能很难在这些物种之间定义明确的遗传边界。

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